|Table of Contents|

Optimization of Pretreatmen Method of Platycodon grandiflorus Seeds Based on PB-CCD Design(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年23
Page:
153-156
Research Field:
Publishing date:

Info

Title:
Optimization of Pretreatmen Method of Platycodon grandiflorus Seeds Based on PB-CCD Design
Author(s):
SUN Xiaochun1; LI Xiaohong2; HU Kunxia2; TANG Zhishu1
（1.Shaanxi University of Chinese Medicine/Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization/Shaanxi Province Key Laboratory of New Drugs and Chinese medicine Foundation Research，Xianyang，Shaanxi 712083；2.College of Pharmacy,Shaanxi University of Chinese Medicine，Xianyang，Shaanxi 712046）
Keywords:
Platycodon grandiflorus seeds; germination; Plackett-Burman design; Central Composite Design; pretreatment
PACS:
-
DOI:
10.11937/bfyy.201623035
Abstract:
Platycodon grandiflorus seeds were used as test materials，the pretreatment method was optimized by Plackett-Burman-Central Composite Design（PB-CCD）.With the seed germination rate as evaluation indexes，the impact of soak time，soak temperature，GA3 concentration，pre-reezing time and solid-liquid ratio on germination rate were analyzed by PB design，and the factors which had significant effect on germination were selected for further optimization based on CCD design.The results showed that the optimum condition of pretreatment of Platycodon grandiflorus seeds was soak 18 hours at 27 ℃ with 3 g?L-1 GA3.Seed germination rate was 92.3% at the optimum model，it indicated that the model was highly predictive and reliable.

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Last Update: 2017-01-18