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Optimization for Extraction Process of Daucus carota Linn Total Flavonoids and Determination of Antioxidant Activity Based on Orthogonal Test(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年23
Page:
134-138
Research Field:
Publishing date:

Info

Title:
Optimization for Extraction Process of Daucus carota Linn Total Flavonoids and Determination of Antioxidant Activity Based on Orthogonal Test
Author(s):
YANG ShenmingWANG ZhenjiFAN ShuguoWANG Cailian
(Department of Chemistry and Life Science,Chuxiong Normal University,Chuxiong,Yunnan 675000)
Keywords:
Daucus carota Linntotal flavonoidsorthogonal test methodultrasonic assisted extractionantioxidant activity
PACS:
-
DOI:
10.11937/bfyy.201623030
Abstract:
Daucus carota Linn was used as test material,total flavonoids was extracted by ultrasound-assisted extraction technology based on orthogonal test,and antioxidant activities on scavenging 1,1-diphenyl-2-picrylhydrazyl radica (DPPH?) and hydroxyl radical (?OH) were studied.The results showed that the optimum extraction parameters were ultrasonic extraction time was 60 minutes,ultrasonic temperature was 40 ℃,ratio of solid to liquid was 1∶50 g?mL-1,and the volume fraction of ethanol was 80%.Under the optimal condition,the average yield of total flavonoids was 27.46%.At a flavonoids concentration of 0.445 mg?mL-1,the percentage scavenging of DPPH? and ?OH were 89.65% and 37.05%.The Daucus carota Linn total flavonoids with good antioxidant activity had the potential to be further developed and utilized as a natural antioxidant.

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Last Update: 2017-01-18