|Table of Contents|

Pathogen Identification and Biological Characteristics of Onion Violet Leaf Spot(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年23
Page:
111-116
Research Field:
Publishing date:

Info

Title:
Pathogen Identification and Biological Characteristics of Onion Violet Leaf Spot
Author(s):
WANG Yong; ZHOU Xuemei; SHENG Jie; SONG Ce; CHEN Long; CHEN Dian
onion violet leaf spot；Alternaria porri (Ell.) Cifferri；mycelial growth；sporulation characteristics
Keywords:
onion violet leaf spot; Alternaria porri (Ell.) Cifferri; mycelial growth; sporulation characteristics
PACS:
-
DOI:
10.11937/bfyy.201623026
Abstract:
The plant of onion violet leaf spot was used as test material which was isolated from onion in three areas of Heilongjiang Province.The pathogen of onion violet leaf spot was isolated by tissue isolation，and the pathogen was identified by morphological and 16s rDNA ITS sequence analysis and the biological characteristics were conducted.The results showed that the pathogen of onion violet leaf spot was Alternaria porri (Ell.) Cifferri.When glucose was used as carbon source，the spore production was the highest，but the amount of mycelium growth was the lowest.When the ammonium chloride was the nitrogen source，the amount of mycelium growth and spore production reached the highest.The mycelium growth was the highest at 32 ℃，pH 7，and the condition was 12 h light 12 h dark alternate culture；the spore production was the highest at 24 ℃，pH 8 under continuous dark conditions.

References:

 

[1]李祥睿.洋葱的营养保健功能与开发利用[J].中国食物与营养,2009(9):55-57.

[2]王芳德.洋葱紫斑病的发生与防治[N].山东科技报，2016-05-09(002).

[3]程智慧,沈永杰.大蒜紫斑病菌的分离及培养条件研究[J].西北农业学报,2008,17(2):274-278.

[4]沈永杰.大蒜紫斑病病菌生物学特性及粗毒素培养条件研究[D].杨凌:西北农林科技大学,2007.

[5]温嘉伟,朱琳,牟喜涛,等.葱紫斑病发生及防治若干问题的初步研究Ⅰ.分生孢子萌发侵染、潜育显症及杀菌剂筛选[J].吉林农业大学学报,2007,29(1):33-36.

[6]董建国.大葱紫斑病的发生及防治方法[J].现代农村科技,2015(20):28.

[7]SAVITHA A S,AJIGHKUMAR K,RAMESH G.Integrated disease management of purple blotch[Alternaria porri (Ellis) Cif] of onion[J].Pest Management in Horticultural Ecosystems,2014,20(1):97-99.

[8]RAO A S,GANESHAN G,RAMACHANDRA Y L,et al.Bioefficacy of fungicides against Alternaria porri (Ellis) Cif.causing purple blotch of onion (Allium cepa L.)[J].Environment and Ecology,2015,33(1):99-104.

[9]方中达.植病研究方法[M].北京:中国农业出版社,1998.

[10]张天宇.中国真菌志[M].北京:科学出版社,2003.

[11]乔镜澄,麻杰.番茄黑斑病病原菌:链格孢菌的分离与鉴定[J].内蒙古农业大学学报(自然科学版),2015(6):91-94.

[12]卢钟彦,梁巧兰,何武学.番茄早疫和晚疫病害病原菌鉴定及室内药剂的筛选[J].草原与草坪,2016(1):83-88.

[13]刘丽,刘长远,汪岐禹.大白菜黑斑病病原鉴定及品种抗性评价[J].辽宁农业科学,2015(1):80-81.

[14]严雪瑞,周源,赵睿杰,等.蓝莓叶穿孔病菌鉴定及其生物学特性研究[J].北方园艺,2014(16):123-127.

[15]范文忠,李进,高郁芳.不同碳氮源对四种链格孢菌生长的影响[J].湖北农业科学,2012,51(9):1795-1797.

[16]马海霞,刘影,屈直,等.芸薹链格孢菌孢子萌发及附着胞形成的初步定量研究[J].吉林农业大学学报,2013,35(4):393-397.

[17]王媛媛,刘彬,周园园,等.玉米链格孢病菌生物学特性及防治药剂离体活性筛选[J].沈阳农业大学学报,2015,46(5):538-542.

[18]周兵,陈洁,马胜平,等.空心莲子草致病菌链格孢菌不同菌株的致病性及培养条件[J].西南农业学报,2016,29(2):321-326.

Memo

Memo:
-
Last Update: 2017-01-18