|Table of Contents|

RAPD and SSR Analyses of Genetic Diversity of Ginseng Germplasm From Different Habitats(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年23
Page:
96-101
Research Field:
Publishing date:

Info

Title:
RAPD and SSR Analyses of Genetic Diversity of Ginseng Germplasm From Different Habitats
Author(s):
HOU Zhifang1; MENG Lulu2; XU Shiquan1; WANG Yingping1; LEI Xiujuan1; ZHENG Peihe1
(1.Institute of Special Wild Economic Animals and Plants，Chinese Academy of Agricultural Sciences，Changchun，Jilin 130112；2.Modern Chinese Medicine and Bio-pharmaceutical Bases in Jilin Province Office，Changchun，Jilin 130112)
Keywords:
ginseng; RAPD; SSR; genetic diversity
PACS:
-
DOI:
10.11937/bfyy.201623023
Abstract:
Taking ginseng germplasms from 15 regions as materials，the genetic diversity of ginseng (Panax ginseng C.A Meyer) produced in 15 regions was analyzed by using RAPD and SSR.The results showed that two kinds of molecular markers could reveal the genetic diversity among different regions.In this paper，11 random RAPD primers were selected，each primer could amplify 3-17 DNA fragments，eleven RAPD primers had amplified 104 clear bands，100 polymorphic bands，and the percentage of polymorphism was 96.15%.RAPD revealed for the test between genetic similarity coefficient (GS) was 0.505 3-0.989 5，average of 0.760 4.Selected five pairs of SSR primers，per pair of primers could amplify DNA fragments from one to eight，a total of 38 clear amplified bands，35 polymorphic bands，percentage of polymorphism was 92.11%，the test revealed the genetic similarity coefficient (GS) was 0.400 0-0.960 0，average of 0.742 1.RAPD and SSR markers constructed cluster diagram was little difference in classification，but the overall trend was consistent.The samples were integrated into three categories by RAPD，SSR and RAPD+SSR，respectively.It could reveal the relationships among them，and laid the theoretical foundation for the introduction and cultivation and breeding of ginseng germplasm.

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Last Update: 2017-01-18