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Rapid Detection of〖STHX〗 Crr3 Gene Against Clubroot Disease by PCR and Selection of Corresponding Germplasm in Chinese Cabbage (Brassica pekinensis L.)(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年22
Page:
120-123
Research Field:
Publishing date:

Info

Title:
Rapid Detection of〖STHX〗 Crr3 Gene Against Clubroot Disease by PCR and Selection of Corresponding Germplasm in Chinese Cabbage (Brassica pekinensis L.)
Author(s):
SUN Zhaohui1; MA Anfeng1; CHENG Fei1; CAO Dandan1; REN Pingping1; GAO Jianwei2
(1.Horticultural College，Qingdao Agriculture University,Qingdao，Shandong 266109;2.Vegetable and Flower Research Institute,Shandong Academy of Agricultural Sciences,Jinan，Shandong 250100)
Keywords:
Chinese cabbage; clubroot disease; Crr3 gene; molecular markers; germplasm
PACS:
-
DOI:
10.11937/bfyy.201622030
Abstract:
The resistant line ‘YM-7’ and the susceptible line ‘Guan 291’，their F1，F2 generations and other 24 germplasms from foreign and domestic companies were used as test materials.Molecular marking method and artificial inoculation method were applied to select effective molecular marker to screen Crr3 gene from two published candidates.The results showed that 1.3 kb amplification fragment in ‘YM-7’,1.0 kb fragment in ‘Guan 291’ and both 1.3 kb and 1.0 kb fragments in their F1 generation were amplified with primer OPC11-2S.The results of artificial test and molecular marker screening for F2 generation of ‘YM-7×Guan 291’ were consistent.The primer OPC11-2S could not only identify the existence of Crr3 gene，but also could test the homozygosis or heterozygosis of Crr3 gene in germplasms accurately.Two germplasms with heterozygous Crr3 resistant gene were manifested.

References:

 

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Last Update: 2017-01-06