Optimization of Tissue Culture and Rapid Propagation of ‘Cabernet Sauvignon’ Grape(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Optimization of Tissue Culture and Rapid Propagation of ‘Cabernet Sauvignon’ Grape

Author(s):: XU Meilong1; 2; LIU Jing1; 2; WANG Qiaozhen1; 2; YUAN Ting1; 2; (1.Ningxia Forestry Institute Co.Ltd.,Yinchuan,Ningxia 750004；2.The National Center of Research and Engineering Technology of Economic Forest Tree Speedy Propagation,Yinchuan,Ningxia 750004)

Abstract:: Wine grape ‘Cabernet Sauvignon’ was used as test material，tissue culture method was used，the effect of different basic culture medium with different concentration of IBA combination and different culture containers on ‘Cabernet Sauvignon’ grape growth was studied，in order to enhance the growth potential of ‘Cabernet Sauvignon’ grape in vitro and improve the survival rate of transplanting.The results showed that the 1/2B5 containing 0.2 mg?L-1 IBA+30 g?L-1 sucrose+7 g?L-1 agar+1 g?L-1 activated carbon was suitable for ‘Cabernet Sauvignon’ grape in vitro culture.‘Cabernet Sauvignon’ grape seedlings grew strongly in the bottle with breathable film diameter of 3.0 cm and light transmission rate 90%.The transplanting survival rate was the highest 96.67%.

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Last Update: 2017-01-06