|Table of Contents|

Development and Utility of EST-SSR Markers in Lagerstroemia indica(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年22
Page:
107-111
Research Field:
Publishing date:

Info

Title:
Development and Utility of EST-SSR Markers in Lagerstroemia indica
Author(s):
ZHANG EnliangWANG PengLI YaWANG Shu′anLI LinfangYANG Rutong
(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing,Jiangsu 210014)
Keywords:
Largerstroemia indicaEST-SSRtranscriptome sequencingmolecular markers
PACS:
-
DOI:
10.11937/bfyy.201622027
Abstract:
Lagerstroemia indica was used as test material,the EST-SSR molecular markers of Lagerstroemia indica were sought from transcriptome sequencing,45 308 unigenes were analyzed by MISA.The results showed that a total of 10 905 EST-SSRs were recognized from 9 074 unigenes,1 486 sequences contained more than one SSR and 550 SSRs presented in compound formation.In this study,a total of 4 501 primer pairs were designed from 7 620 SSRs.To validate SSR markers,68 primer pairs were randomly selected to PCR amplification and 61 SSR markers were amplified successfully.Of these validated SSR markers,28 primer pairs were detected polymorphism.

References:

 

[1]CABRERA R I.Evaluating and promoting the cosmopolitan and multipurpose Lagerstroemia[J].Acta Horticulturae2004630:177-184.

[2]ZHANG Q.Studies on cultivars of crapemyrtle(Lagerstroemia indica)and their uses in urban greening[J].Journal of Beijing Forestry University1991(4):57-66.

[3]MIN WPING SXIANG R Xet al.Recent advances in Lagerstroemia indica resources and breeding[J].Shandong Forestry Sci Technol2008,266-68.

[4]GUPTA P KVARSHNEY R K.The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat[J].Euphytica2000113(3)163-185.

[5]BOZHKO MRIEGEL RSCHUBERT Ret al.A cyclophilin gene marker confirming geographical differentiation of Norway spruce populations and indicating viability response on excess soil-born salinity[J].Molecular Ecology200312(11)3147-3155.

[6]KAWASE DUENO STSUMURA Yet al.Development and characterization of EST-SSR markers for Sciadopitys verticillata (Sciadopityaceae)[J].Conservation Genetics200910(6)1997-1999.

[7]WU JCAI CCHENG Fet al.Characterisation and development of EST-SSR markers in tree peony using transcriptome sequences[J].Molecular Breeding201434(4)1853-1866.

[8]CHENG YYANG YWANG Zet al.Development and characterization of EST-SSR markers in Taxodium Zhongshansa[J].Plant Molecular Biology Reporter201533(6)1804-1814.

[9]WANG XWADL P APOUNDERS Cet al.Evaluation of genetic diversity and pedigree within crape myrtle cultivars using simple sequence repeat markers[J].Journal of the American Society for Horticultural Science2011136(2)116-128.

[10]CAI MPAN H TWANG X Fet al.Development of novel microsatellites in Lagerstroemia indica and DNA fingerprinting in Chinese Lagerstroemia cultivars[J].Scientia Horticulturae2011131(1):385-394.

[11]LIU YHE DCAI Met al.Development of microsatellite markers for Lagerstroemia indica (Lythraceae) and related species[J].Applications in Plant Sciences20131(2):76-87.

[12]DOYLE J.Isolation of plant DNA from fresh tissue[J].Focus199012:13-15.

[13]LI YZHANG ZWANG Pet al.Comprehensive transcriptome analysis discovers novel candidate genes related to leaf color in a Lagerstroemia indicayellow leaf mutant[J].Genes and Genomics201537(10):851-863.

[14]JUNG SABBOTT AJESUDURAI Cet al.Frequencytypedistribution and annotation of simple sequence repeats in Rosaceae EST[J].Functional and Integrative Genomics20055(3):136-43.

[15]JAIN AGHANGAL RGROVER Aet al.Development of EST-based new SSR markers in seabuckthorn[J].Physiology & Molecular Biology of Plants an International Journal of Functional Plant Biology201016(4):375-8.

[16]PENG ZGALLO MTILLMAN B Let al.Molecular marker development from transcript sequences and germplasm evaluation for cultivated peanut (Arachis hypogaea L.)[J].Molecular and General Genetics2015291(1):1-19.

[17]CARDLE LRAMSAY LMILBOURNE Det al.Computational and experimental characterization of physically clustered simple sequence repeats in plants[J].Genetics2000156(2):847-854.

[18]LOW E T LALIAS HBOON S Het al.Oil palm Elaeis guineensis Jacq.) tissue culture ESTsIdentifying genes associated with callogenesis and embryogenesis[J].BMC Plant Biology20088(3)62.

[19]QIU LYANG CBO Tet al.Exploiting EST databases for the development and characterization of EST-SSR markers in castor bean Ricinus communis L.)[J].BMC Plant Biology201010(4):1-10.

[20]YADAV H KRANJAN AASIF M Het al.EST-derived SSR markers in Jatropha curcas L.developmentcharacterizationpolymorphismand transferability across the species/genera[J].Tree Genetics and Genomes20117(1):207-219.

[21]KUMARI MGROVER AYADAV P Vet al.Development of EST-SSR markers through data mining and their use for genetic diversity study in Indian accessions of Jatropha curcas L.A potential energy crop[J].Genes and Genomics201335(5):661-670.

[22]CHEN CPING ZCHOI Y Aet al.Mining and characterizing microsatellites from citrus ESTs[J].Theoretical and Applied Genetics2006112(7):1248-1257.

[23]GAO CTANG ZYIN Jet al.Characterization and comparison of gene-based simple sequence repeats across Brassica species[J].Molecular Genetics and Genomics2011286(2):161-170.

[24]WANG RXU SJIANG Yet al.De novo sequence assembly and characterization of Lycoris aurea transcriptome using GS FLX titanium platform of 454 pyrosequencing[J].PLoS One20138(4):e60449.

[25]FENG S PLI W GHUANG H Set al.Developmentcharacterization and cross-species/genera transferability of EST-SSR markers for rubber tree Hevea brasiliensis)[J].Molecular Breeding200923(1):85-97.

[26]FUJIMORI SWASHIO THIGO Ket al.A novel feature of microsatellites in plantsa distribution gradient along the direction of transcription[J].Febs Letters2003554(1-2):17-22.

[27]VARSHNEY R KGRANER ASORRELLS M E.Genic microsatellite markers in plantsfeatures and applications[J].Trends in Biotechnology200523(1):48-55.

[28]GROVER ASHARMA P C.Microsatellite motifs with moderate GC content are clustered around genes on Arabidopsis thaliana chromosome 2[J].Silico Biology20077(2):201-213.

[29]GROVER AAISHWARYA VSHARMA P C.Biased distribution of microsatellite motifs in the rice genome[J].Molecular Genetics and Genomics 2007277(5):469-480.

[30]GROVER ASHARMA P C.Occurrence of simple sequence repeats in potato ESTs is not randomAn in silico study on distribution and length of simple sequence repeats[J].Potato J200431:95-102.

[31]KUMPATLA S PMUKHOPADHYAY S.Mining and survey of simple sequence repeats in expressed sequence tags of dicotyledonous species[J].Genome,200548(6):985-498.

Memo

Memo:
-
Last Update: 2017-01-06