Plant Regeneration From Leaves of Seedless Date Plum (Diospyros lotus L.)(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Plant Regeneration From Leaves of Seedless Date Plum (Diospyros lotus L.)

Author(s):: ZHOU Ruijin; ZHANG Xiaona; HU Huiling; LI Guirong; SONG Ruhui; （School of Horticulture and Landscape Architecture，Henan Institute of Science and Technology，Xinxiang，Henan 453003)

Keywords:: date plum（Diospyros lotus L.）; leaf culture; plantlet regeneration

Abstract:: Taking seedless Diospyros lotus L.‘Zanyuan wuhe’ as test material，factors affecting in vitro leaf regeneration including basal medium，plant growth regulator and dark period were studied.The results showed that a high efficient system of in vitro leaf regeneration was established.The superior induction medium was (1/2N)MS+5.0 mg?L-1 ZT+0.10 mg?L-1 IAA，dark incubated for the first four weeks and then transferred to light was the most optimum for plant regeneration，and the callus percentage，the shoot percentage and the adventitious buds per leaf disc were 99.1%,87% and 5.4,respectively.(1/2N)MS+0.5 mg?L-1 ZT+0.01 mg?L-1 IAA was suitable for subculture of shoot.The regenerated plantlets rooted well in 1/2MS+1.0 mg?L-1 IAA+20 g?L-1 sugar+7 g?L-1 agar，of which rooting percentage was 60.5%.The survival rate of rooted plantlets in the field was 63%.

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Last Update: 2017-01-06