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Influence of Hydrogen Peroxide Traps on Antioxidant System in Different Organs of Dianthus chinensis L.Under H2O2 Treatment(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年22
Page:
82-86
Research Field:
Publishing date:

Info

Title:
Influence of Hydrogen Peroxide Traps on Antioxidant System in Different Organs of Dianthus chinensis L.Under H2O2 Treatment
Author(s):
ZHANG QianqianYU XiaoHE Xueqin
(Agronomy Faculty,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010019)
Keywords:
Dianthus chinensis L.hydrogen peroxidedimethylthioureadiphenyleneiodonium chloridescavenger enzymes
PACS:
-
DOI:
10.11937/bfyy.201622021
Abstract:
Taking Dianthus chinensis L.as material,with 25 mmol?L-1 DMTU and 3 mmol?L-1 DPI for 2 hours were pretreated and 5 mmol?L-1 H2O2 for 6 hours were treated,then the contents of H2O2 and O?[TX-*4]2and related enzyme activity were measured,the influence of reducing H2O2 content on physiological activity under oxidative stress in Dianthus chinensis L.seedlings were studied,in order to provide theoretical basis for cultivation of Dianthus chinensis L.under adversity.The results showed that the contents of H2O2 and O?[TX-*4]2 in the leaves and roots were reduced by pretreatment of 25 mmol?L-1 DMTU and 3 mmol?L-1 DPI under normal conditions and levels of reduce were significant in the roots (P<0.5);treatment with 5 mmol?L-1 H2O2 led to the significant increase of the content of H2O2 in leaves and the content of O?[TX-*4]2in roots (P<0.5),but pretreatment with 25 mmol?L-1 DMTU and 3 mmol?L-1 DPI declined both in the two organs;the enzyme activities of SOD in leaves and SOD,APX in roots increased significantly under 5 mmol?L-1 H2O2 treatment (P<0.5),but the enzyme activities of SOD and APX in leaves were declined by pretreatments of 25 mmol?L-1 DMTU and 3 mmol?L-1 DPI and the enzyme activity of APX in roots was significantly declined by pretreatments of 25 mmol?L-1 DMTU (P<0.5),it suggested that the contents of H2O2 and O?[TX-*4]2 had positive relations with the activities of their scavenger enzymes.Therefore,DMTU and DPI could alleviate the accumulation of H2O2 and O?[TX-*4]2content induced by H2O2 treatment with different levels,and the former had the better effect of alleviation.

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Last Update: 2017-01-06