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Extraction of Soluble Solids From Fruit Body Feet of Flammulina velutipes and Pleurotus eryngii by Enzymatic Hydrolysis(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年21
Page:
155-159
Research Field:
Publishing date:

Info

Title:
Extraction of Soluble Solids From Fruit Body Feet of Flammulina velutipes and Pleurotus eryngii by Enzymatic Hydrolysis
Author(s):
LIN Qunying1; LONG Liangkun2; GE Tingting2; ZHANG Fenglun1; XIANG Chunrong3; SUN Xiaoming1
(1.Nanjing Institute for the Comprehensive Utilization of Wild Plants,Nanjing,Jiangsu 210042；2.College of Chemical Engineering，Nanjing Forestry University，Nanjing,Jiangsu 210037；3.Zhenjiang City Dantu District Zhengdong Ecological Agriculture Development Center，Zhenjiang，Jiangsu 212000)
Keywords:
Flammulina velutipes; Pleurotus eryngii; fruit body feet; soluble solids; enzymatic hydrolysis
PACS:
-
DOI:
10.11937/bfyy.201621038
Abstract:
Fruit body feet of Flammulina velutipes and Pleurotus eryngii were used as test materials，single factor tests and orthongonal design experiments were applied to optimize the enzymatic hydrolysis conditions for extraction of soluble solids.The components and antioxidant activity of the hydrolysate were studied,to provide reference for exploration of hydrolysates from fruit body feet of F.velutipes and P.eryngii. The results showed that edible mushroom hydrolysis enzyme at 1.5%，flavor enzyme at 1.5% and material to water ratio at 1∶30 g?mL-1 consisted the best conditions for extratction of soluble solids.The hydrolysis products of fruit body feet of F.velutipes and P.eryngii respectively contained 24.35% and 12.30% of crude protein，35.92% and 37.40% of crude polysaccharides，0.77 mg? kg-1 and 0.64 mg?kg-1 of flavones，4.82 mg? kg-1 and 4.18 mg?kg-1 of vitamin C.It was proved that hydrolysis products poccessed good anti-oxidant activities.Hydrolysis products of F.velutipes at low concentration (0.1 mg?mL-1) could obviously improve the antioxidant capacity of the nematode，extending the longest survival time from 5 days (CK) to 12 days.And the antioxidant ability of hydrolysate of P.eryngii was positively correlated with the concentration.The longest survival time and mean lifespan were both extended as concentrations increased.At high concentration of 2.0 g?L-1,the longest survival time and mean lifespan could reach to 16 days and 8.27 days，respectively.

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Last Update: 2017-01-03