Study on Polysaccharides in the Fruit Body of Hericium erinaceus Cultivated With JUNCAO(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on Polysaccharides in the Fruit Body of Hericium erinaceus Cultivated With JUNCAO

Author(s):: CAI Yangxing1; 2; CAO Xiuming1; 2; LIN Dongmei1; 2; LUO Hailing1; 2; LIN Hui1; 2; LIN Zhanxi1; 2; (1.National Engineering Research Center of JUNCAO Technology，Fujian Agriculture and Forestry University，Fuzhou，Fujian 350002；2.JUNCAO Research Institute，Fujian Agriculture and Forestry University，Fuzhou，Fujian 350002)

Keywords:: Hericium erinaceus; polysaccharide; optimum condition of extraction; separation and purification

Abstract:: Using the fruit bodies of Hericium erinaceus which cultivated with JUNCAO and sawdust as test materials，the effect of coarse polysaccharide′s extraction rate from two kinds of fruit bodies on extraction condition such as extraction times，ratio of solid material to liquid，extraction time and temperature were investigated by L9（34）orthogonal experimental design method；the coarse polysaccharides were isolated and purified preliminary by DEAE SepHarose Fast Flow column chromatography method and SepHadex G-100 Gel chromatography method.Some components with single symmetrical peak were expected to obtain.The results showed that the optimize extract conditions of Hericium erinaceus fruit body′s polysaccharides were extract two times，ratio of material to water 1∶25 g?mL-1，time of 4 hours and temperature of 90 ℃.Three components were obtained from Hericium erinaceus fruit body′s polysaccharide under JUNCAO cultivation，there were cHEP1，cHEP2 and cHEP3；and only two components were abtained from the polysaccharide under traditional sawdust cultivation，there were sHEP1 and sHEP2.Purity identification showed that they were all single symmetrical peak.

[1]徐锦堂.中国药用真菌学[M].北京:北京医科大学和中国协和医科大学联合出版社,1997:448-449.

[2]黄年来,林志彬,陈国良.中国食药用菌学[M].上海:上海科学技术文献出版社,2010(下):739-750.

[3]樊伟伟,黄惠华.猴头菇多糖研究进展[J].食品科学,2008(1):355-358.

[4]刘梅森,陈海晏,孙红斌.猴头菌的药用价值概述[J].中国食用菌,1999,18(1):24-25.

[5]赵金芬.猴头菌菌丝多糖提取工艺的研究[J].中国医药导刊,2009,11(7):1242-1243.

[6]崔玉海.猴头菌多糖的分离纯化及活性探讨[J].黑龙江医药科学,2004,27(4):18-19.

[7]杜志强,王建英.猴头菌菌丝多糖的分离纯化及其性质研究[J].食品研究与开发,2011(10):128-130.

[8]林占熺.菌草学[M].3版.北京:国家行政学院出版社,2013:285-292.

[9]国家技术监督局.GB/T12530-199食用菌取样方法[S].北京:中国标准出版社,1990.

Last Update: 2017-01-03