|Table of Contents|

Construction and Evaluation of Yeast Two Hybrid cDNA Library for Eggplant Root Inoculation With R.solanacearum(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年21
Page:
102-105
Research Field:
Publishing date:

Info

Title:
Construction and Evaluation of Yeast Two Hybrid cDNA Library for Eggplant Root Inoculation With R.solanacearum
Author(s):
XIAO Xi′ou12LIN Wenqiu12LI Wei12GAO Xiaomin12LYU Lingling12
(1.South Subtropical Crop Research Institute,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang,Guangdong 524091;2.Construction on the Research and Development Center of Industry Technology for Provincial Modern Agriculture (Key Tropical Crops),Zhanjiang,Guangdong 524091)
Keywords:
eggplantR.solanacearumyeast two hybridscDNA library
PACS:
-
DOI:
10.11937/bfyy.201621026
Abstract:
Taking ‘Q31-1’ eggplant as test material,the yeast two hybrid cDNA library was constructed after the eggplant inoculation with R.solanacearum,in order to research the interaction mechanism between the eggplant and R.solanacearum.The results showed that the library contained 1.25×107 CFU with 100% recombination rate based on 24 randomly selected clones.The result of PCR showed that length of insert cDNA fragments was greater than 1.5 kb.The results demonstrated that the library could be used to screen the interaction protein.

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Last Update: 2016-12-30