|Table of Contents|

SRAP Analysis of Germplasm Resources of Wild Astragalus membranaceus in Changbai Moutain(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年21
Page:
94-97
Research Field:
Publishing date:

Info

Title:
SRAP Analysis of Germplasm Resources of Wild Astragalus membranaceus in Changbai Moutain
Author(s):
WANG Meng1WANG Jingran1LI Deyang2QUAN Xueli1WU Songquan1
(1.Agricultural College,Yanbian University,Yanji,Jilin 133002;2.Landscape Engineering Limited Company of Meicheng of Jilin Province,Changchun,Jilin 130022)
Keywords:
Astragalus membranaceusSRAPgenetic diversitygermplasm
PACS:
-
DOI:
10.11937/bfyy.201621024
Abstract:
Taking leaves of wild Astragalus membranaceus as test materials,genetic diversity of germplasm resources of wild A.membranaceus in Changbai Moutain was studied by using SRAP molecular markers,in order to provide a foundation for preserving and breeding good varieties for Astragalus membranaceus.The results showed that 16 pairs of primers,which could produce more,characteristic and reproducible bands,were screened from 300 pairs of SRAP primers;these primes amplified 129 bands from which polymorphic bands were 95 bands.The cluster analysis disclosed that there were abundant genetic differences among the wild resources of A.membranaceus in Changbai Moutain,which was beneficial for cross-breeding of A.membranaceus.

References:

 

[1]卢彦琦,贺学礼.黄芪研究现状[J].邯郸农业高等专科报,2004(4):19-25.

[2]王树斌,全雪丽,周玉红,.膜荚黄芪子叶节植株再生体系的建立[J].北方园艺,2012(6)177-178.

[3]国家药典编委会.《中华人民共和国药典》[M].北京:中国医药科技出版社,2010:8.

[4]赵明,黄文哲.中国黄芪属药用植物资源现状及分析[J].中国野生植物资源,2010,19(6):5-9.

[5]李淑芳.中药黄芪药理作用研究进展[J].湖北中医杂志,2013(6):73-75.

[6]赵明,黄文哲.中国黄芪属药用植物资源现状及分析[J].中国野生植物资源,2000,19(6):5-9.

[7]毛伟,李培英,孙宗玖.偃麦草SRAP-PCR反应体系优化[J].北方园艺,2015(2)91-97.

[8]柳李旺,龚义勤,黄浩,.新型分子标记:SRAPTRAP及其应用[J].遗传,2004,26(5)777-781.

[9]LI GQUIROS C F.Sequence-related amplified polymorphism (SRAP)a new marker system based on a simple PCR reactionits application to mapping and gene tagging in Brassica[J].Theoretical and Applied Genetics2001,103(2):455-461.

[10]赵英,黄国弟,付海天,.十二份杧果种植亲缘关系的SRAP分析[J].北方园艺,2015(21)99-102.

[11]涂勋良.36个石榴品种的SRAP遗传多样性研究[D].雅安:四川农业大学,2012:3-5.

[12]潘祖桐.SRAPRSAP在侧耳属菌种亲缘关系分析中的研究[D].长春:吉林农业大学,2012.

[13]王珍,方宣钧.植物DNA分离[J].分子植物育种,2003,1(2):281.

[14]金美玉,廉美兰,吴松权,.黄芪SRAP反应体系优化[J].江苏农业科学,2009(5):21-22.

[15]巩檑,石磊,甘晓燕,.冬枣SRAP扩增体系的优化[J].西北农业学报,2013,22(4):109-113.

[16]杨浩.基于SPSS的聚类分析在行业统计数据中的应用[D].长春:吉林大学,2013.

[17]BUDAK HSHEARMAN R CPARMAKSIZ Iet alComparative analysis of seeded and vegetative biotype buffalo grasses based on phylogenetic relationship using ISSRsSSRsRAPDs and SRAPs[J].Theoretical and Applied Genetics2004109280-288.

Memo

Memo:
-
Last Update: 2016-12-30