|Table of Contents|

Transformation Techniques for Petunia hybrida With ACC Deaminase Gene Driven by Different Promoters(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年20
Page:
85-91
Research Field:
Publishing date:

Info

Title:
Transformation Techniques for Petunia hybrida With ACC Deaminase Gene Driven by Different Promoters
Author(s):
LI Jianyong12
(1.College of Jiasixie Agriculture,Weifang University of Science and Technology,Shouguang,Shandong 262700;2.Laboratory of Plant Improvement and Utilization,Yunnan University,Kunming,Yunnan 650091)
Keywords:
Petunia hybrida promoterACC deaminase genegenetic transformation
PACS:
-
DOI:
10.11937/bfyy.201620023
Abstract:
Taking Petunia hybrida as material,ACC deaminase gene(ACDS) (driven respectively by promoter CaMV35S,SAG12 and CHSA) was transformed into Petunia hybrida leaves,mediated by Agrobacterium tumefaciens.The effects of the pre-culture time,concentration of Agrobacterium tumefaciens,infection time,co-culture time and concentration of AS on transformation were studied.The transformation efficiency of ACDS gene driven by different promoters was compared based on the percentage of transgenic plants and the growth rate of transgenic plants.The results showed that the best transformation efficiency was abtained under the conditions of Petunia hybrida leaves pre-culture for 5 days,infection for 8 minutes in Agrobacterium tumefaciens liquid of 0.6 OD600 value containing 200 μmol?L-1 acetosyringone,and then co-culture for 4 days.ACDS gene driven by CHSA promoter had the highest transformation efficiency(3%),while ACDS gene driven by SAG12 promoter had the lowest transformation efficiency(1.25%).PCR analysis verified that the foreign gene was integrated into the Petunia hybrida genome.The obtaining of Petunia hybrida transgenic plants set up a technical basis for breeding of anti-aging Petunia hybrida varieties via genetic engineering.

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Last Update: 2016-11-22