|Table of Contents|

Culture Conditions of Protocorms Proliferation of Cremastra appendiculata(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年19
Page:
124-128
Research Field:
Publishing date:

Info

Title:
Culture Conditions of Protocorms Proliferation of Cremastra appendiculata
Author(s):
WU YanqiuLYU XiangLI XiaolanGAO XiaofengLIU JiandongZHANG Mingsheng
(School of Life Sciences,Guizhou University/Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education),Guiyang,Guizhou 550025)
Keywords:
Cremastra appendiculata (D.Don.) Makinoprotocormsproliferation culture
PACS:
-
DOI:
10.11937/bfyy.201619031
Abstract:
The protocorms from the germination of Cremastra appendiculata seed were used as test materials to investigate the effects of the media,growth regulators(6-BA,NAA and IBA),active carbon (AC),temperature and light intensity on protocorms proliferation of C.appendiculata.The results showed that 1/2 MS medium was the appropriate basic medium for protocorms proliferation.Adding 6-BA,NAA and IBA into 1/2 MS medium was beneficial to protocorms proliferation.The proliferation rate reached to 170.07% after 40 days while adding 1.0 mg?L-1 IBA,and adding certain amount of AC could promote the proliferation.In addition,the temperature and light intensity were also necessary to the proliferation.In conclusion,the suitable culture condition for protocorms proliferation of C.appendiculata was 1/2 MS adding 1.0 mg?L-1 6-BA,1.0 mg?L-1 IBA and 0.5 g?L-1 AC with 15 ℃ and 2 000 lx light intensity.

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Last Update: 2016-11-08