Research on the Identification and Biological Characteristics of Late Blight Pathogen of Greenhouse Tomato in Tibet(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Research on the Identification and Biological Characteristics of Late Blight Pathogen of Greenhouse Tomato in Tibet

Author(s):: XIANG Dong1; DAI Wan’an1; GONG Wenfeng2; YANG Jie1; Nimayuzhen1; （1.Institute of Vegetable，Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa,Tibet 850032；2.College of Agricultural and Animal Husbandry，Tibet University,Linzhi,Tibet 860000）

Keywords:: tomato; Phytophthora infestans; identification; separation; biological characteristics

Abstract:: With typical symptom of late blight of tomato disease leaves as test material，the pathogen was isolated by using the method of leaf claping with potato slices.The effects of different medium，pH，temperature，carbon source and nitrogen source on the mycelium growth and spore production were studied.Through pathogen identification and biological characteristics research，in order to provide a theoretical basis for the occurrence and control of late blight pathogen of greenhouse tomato in Tibet.The results showed the main pathogeny causing tomato late blight was identified as Phytophthora infestans(Mont) de Bary.Most of culture medium were conducive to the growth of mycelium，and RSA was the best one.The optimum temperature for mycelial growth and sporulation was 18 ℃，and the optimum pH was 6-7.The soluble starch was the best C source and maltose was the worst C source.Proline was the best N source while the mycelium was not grown on arginine，cystine and ammonium chloride.The sporangia lethal temperature was 55 ℃,10 min.

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Last Update: 2016-11-08