|Table of Contents|

Characteristics and Expression Analysis of Two CCCH Zinc Finger Genes SlC3H64 and SlC3H65 in Tomato(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年19
Page:
110-114
Research Field:
Publishing date:

Info

Title:
Characteristics and Expression Analysis of Two CCCH Zinc Finger Genes SlC3H64 and SlC3H65 in Tomato
Author(s):
XU Ruirui; CAO Cuiyu
(College of Biological and Agricultural Engineering，Weifang University/Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong，Weifang，Shandong 261061)
Keywords:
tomato; CCCH zinc finger proteins; gene; tissues; stress treatment; expression analysis
PACS:
-
DOI:
10.11937/bfyy.201619028
Abstract:
Taking ‘Zhongshu No.6’ as test material，using the methods of bioinformatics and quantitative RT-PCR，protein structure of SlC3H64 and SlC3H65 CCCH zinc finger proteins were analyzed，several known stress responsive elements in the promoter of these two CCCH zinc finger genes were identified，the expression of these two CCCH zinc finger genes was analyzed in different vegetative organs and under five different stress treatments.The results showed that two CCCH zinc finger proteins had the almost completely different protein tertiary structures.Two CCCH zinc finger genes contained eleven and seven different stress response element，respectively.These elements were different between SlC3H64 and SlC3H65 CCCH zinc finger genes，except the TC-rich repeat element.The existence of stress-related elements in CCCH zinc finger gene promoter regions provided further evidence in future research.Expression analysis showed that two CCCH zinc finger genes expressed in root，stem and leaf in ‘Zhongshu No.6’，with the highest expression level in leaf，similarly.Under five different stress conditions，SlC3H64 was up-regulated under SA and high temperature stress treatment，while SlC3H65 only responsed to mannitol treatment.

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Last Update: 2016-11-08