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The Oxidative Damage of Spirodela polyrrhiza Under Mn Stress(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年19
Page:
89-96
Research Field:
Publishing date:

Info

Title:
The Oxidative Damage of Spirodela polyrrhiza Under Mn Stress
Author(s):
ZHANG Lifang1; WANG Jinghua1; SHI Guoxin1; YANG Haiyan2
(1.College of Life Science,Nanjing Normal University/Jiangsu Key Laboratory of Biodiversity and Biotechnology，Nanjing,Jiangsu 210023；2.Jiangsu Province Institute of Botany,Chinese Academy of Sciences,Nanjing，Jiangsu 210023)
Keywords:
Mn; Spirodela polyrrhiza; oxidative damage; fourier transform infrared spectroscopy（FTIR）
PACS:
-
DOI:
10.11937/bfyy.201619024
Abstract:
The effect of Mn (0，0.02，0.04，0.06，0.08，0.10 mmol?L-1) in different concentrations in the Spirodela polyrrhiza was studied，and the oxidative damage，antioxidant system and the influence of Ca2+-ATP enzyme activity in the S.polyrrhiza were analyzed.The change of the infrared spectra was analyzed by Fourier transform infrared spectroscopy (FTIR).The results showed that Mn stress caused obvious oxidative damage,such us the dramatical increase of the generation rate of O?[TX-*4]2，the accumulation of H2O2 and MDA in great quantities，and the obvious decreases of the content of soluble protein and photosynthetic pigment in leaves.The content of the soluble sugar decreased after an increase in short.The antioxidant system was destroyed.Along with the increase of Mn concentration，the activity of catalase (CAT) and superoxide dismutase (SOD) declined after increasing.But the activity of peroxidase (POD) in leaves increased gradually，and the levels of GSH，vitamin C and vitamin E increased sharply.The content of proline increased obviously.The activity of the Ca2+-ATP enzyme obviously decreased after increasing.The infrared spectra showed that the peak increased firstly and then decreased in the place of 3 396 cm-1，2 920 cm-1，1 658 cm-1 and 1 062 cm-1,which reflected the content of amino acid，sugars，proteins and carboxylic acid were obviously effected by the Mn stress.In a conclusion，oxidative damage of the S.polyrrhiza was obvious under Mn treatment.The balance of antioxidant system has been broken.The activity of the Ca2+-ATP enzyme changed obviously.In addition，the content of amino acids，sugars，carboxylic acid were also significantly affected.In other words，S.polyrrhiza was obviously damaged by the Mn stress.

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Last Update: 2016-11-07