Establishment a Common Stable Salt-tolerance Cell Lines of Sunflower (Helianthus annuus L.)(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Establishment a Common Stable Salt-tolerance Cell Lines of Sunflower (Helianthus annuus L.)

Author(s):: GAO Jinqiu1; SONG Yang1; SUN Lijuan1; SUN Li’na1; ZHANG Yi2; (1.College of Life Sciences,Baicheng Normal University,Baicheng，Jilin 137000;2.Sunflower Institute,Baicheng Academy of Agricultural Sciences，Baicheng，Jilin 137000)

Abstract:: Taking 10 varieties of Helianthus annuus L.as test materials，using the cell suspension technology，the optimum conditions for cultivation of salt-tolerant cell lines were studied.The results showed that cutting out the seven-day sunflower′s cotyledons and hypocotyls and inoculating in 6-BA 05 mg?L-1+NAA 05 mg ?L-1+NaCl 50 mmol?L-1+sucrose 40 g?L-1，pH 55-70 MS medium and culturing in temperature at 28 ℃ for two weeks.Then cutting 1 g the callus and put them in the 30 mL liquid medium which contained NaCl 175 mmol?L-1，shaking and subculturing every seven days，the stable salt-tolerant cell lines were obtained.This scheme was not only applicable for oil sunflower but also for eating sunflower.

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Last Update: 2016-10-31