|Table of Contents|

Clone and Express Analysis of ClCOL〖STBX〗5a in Chrysanthemum lavandulifolium(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年18
Page:
103-107
Research Field:
Publishing date:

Info

Title:
Clone and Express Analysis of ClCOL〖STBX〗5a in Chrysanthemum lavandulifolium
Author(s):
CHEN Dongliang12LUO Chang12CHENG Xi12HUANG Conglin12
(1.Beijing Agro-biotechnology Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097;2.Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology,Beijing 100097)
Keywords:
ChrysanthemumConstans like geneflowering regulationexpress analysis
PACS:
-
DOI:
10.11937/bfyy.201618025
Abstract:
Taking Chrysanthemum as test material,the ClCOL〖STBX〗5a gene was cloned and analyzed.The results showed that this gene encoded a protein of 347 amino acids containing two B-Box domains and one CCT domain,which indicated that it belonged to the CO family group I.Both the results of NCBI Blastp and phylogenetic tree based on CO proteins showed it was mostly closed to AtCOL〖STBX〗5 gene than other member of the family,so it was named as ClCOL〖STBX〗5a.Relative real time PCR analysis showed that ClCOL〖STBX〗5a expressed in all the tested tissues,with the highest level in leaf.In the process of flowering,the expression of ClCOL〖STBX〗5a gene was gradually increased,and reached the maximum level at pigmented stage,and then decreased gradually,which indicated that ClCOL〖STBX〗5a might play an important role in the development of flower in Chrysanthemum.

References:

 

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Last Update: 2016-10-31