|Table of Contents|

Agrobacterium-mediated CBF Genetic Transformation of Hami Zizyphus jujuba(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年18
Page:
94-98
Research Field:
Publishing date:

Info

Title:
Agrobacterium-mediated CBF Genetic Transformation of Hami Zizyphus jujuba
Author(s):
GAO Qiming12WANG Bin2Saimaiti?TUERXUN2XU Ming3LUO Shuping3
(1.Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou,Henan 450009;2.Technology Extension Center of Hami Prefecture Forestry and Fruit Industry,Hami,Xinjiang 839000;3.College of Agronomy,Xinjiang Agricultural University,Urumqi,Xinjiang 830052)
Keywords:
Hami Zizyphus jujubaCBF geneAgrobacterium-mediated
PACS:
-
DOI:
10.11937/bfyy.201618023
Abstract:
Hami Zizyphus jujuba leaves were used as test materials to study genetic transformation of Hami Zizyphus jujuba by using CBF transcription factor of antifreeze gene,the CBF transcription factor of antifreeze gene was induced by using Agrobacterium tumefactions LBA4404,the transgenic plants were identified by using PCR and RT-PCR.The results showed that the highest transformation efficiency was obtained when leaf explants were cultured 3 days after pre-cultured for 2 days and infested for 20 minutes with Agrobacterium tumefactions (OD600=05).7 resistance regenerated transformation systems were obtained in this experiment.CBF genes were positive by using PCR identify and expressed by using RT-PCR analysis in 7 resistance regenerated transformation systems.

References:

 

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Last Update: 2016-10-31