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Study on the Anti-browning Method in Tissue Culture of Taxus chinensis(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年17
Page:
111-114
Research Field:
Publishing date:

Info

Title:
Study on the Anti-browning Method in Tissue Culture of Taxus chinensis
Author(s):
YANG Wenting; KUANG Qian
（College of Environmental and Biological Engineering，Wuhan Technology and Bussiness University,Wuhan，Hubei 430065）
Keywords:
Taxus chinensis; callus; induction rate of callus; browning
PACS:
-
DOI:
10.11937/bfyy.201617026
Abstract:
With the yew axillary buds as explants，B5 as basic culture medium，the effect of different combinations of hormone and additives on browning rate in tissue culture of Taxus was studied.The results showed that under the hormone concentration of 2,4-D 2.0 mg?L-1 and 6-BA 0.5 mg?L-1，the callus grown well，whose contamination rate was 11.11%，the induction rate of callus was 88.89%，browning degree was lower，and PPO was 8.03 U?g-1?min-1.Furthermore，by adding the combination of activated carbon and citric acid，the degree of browning reduced significantly，and PPO was 1.40 U?g-1?min-1.

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Last Update: 2016-10-14