|Table of Contents|

Establishment of Leaf Regeneration System of Crassula arborescens(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年17
Page:
107-110
Research Field:
Publishing date:

Info

Title:
Establishment of Leaf Regeneration System of Crassula arborescens
Author(s):
LI QingweiHOU DianmingSHEN Shunxian
(College of Horticulture and Landscape Architecture,Henan Vocational College of Agriculture,Zhengzhou,Henan 451450)
Keywords:
Crassula arborescensleafadventitious budsregeneration
PACS:
-
DOI:
10.11937/bfyy.201617025
Abstract:
Taking Crassula arborescens leaves as materials,using plant tissue culture method,the effects of sterilizing time and different concentration of 2,4-D,6-BA,NAA on regeneration of Crassula arborescens were studied.The results showed that soaking the leaf in 70%-75% alcohol for 10-15 seconds,then 0.1% HgCl2 disinfection 12-15 minutes,the callus had low contamination rate and high survival rate.MS+1.5 mg?L-1 6-BA+0.5 mg?L-1 2,4-D was optimum medium for inducing adventitious buds with an average of 13.7 adventitious buds;adventitious buds in MS+6-BA 1.5 mg?L-1+NAA 0.1 mg?L-1+GA3 0.5 mg?L-1,the proliferation coefficient was 11.0;multiple shoots could produce an average of 9-11 roots in the 1/2MS+IBA 0.5 mg?L-1 or MS+IBA 0.5 mg?L-1 medium.

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Last Update: 2016-10-14