Establishment of Protein Mulberry Leaves’ Regeneration System and Sensitivity Test of Phosphinothricin(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Establishment of Protein Mulberry Leaves’ Regeneration System and Sensitivity Test of Phosphinothricin

Author(s):: XIONG Yi; WANG Honghong; ZHANG Fangsong; MU Jianqiang; ZHU Jianbo; （College of Life Science，Shihezi University，Shihezi，Xinjiang 832003）

Abstract:: Taking young protein mulberry leaves as explants，MS as the basic medium，the effect of plant growth regulator with different concentrations on the protein mulberry regeneration was studied.The results showed that the best medium of leaf induce callus was MS+TDZ 1.0 mg?L-1+NAA 0.1 mg?L-1；the best differentiation medium was MS+TDZ 0.20 mg?L-1+NAA 0.09 mg?L-1，and the bud differentiations rate was 21.65%；medium of 1/2 MS+PVP 0.2 g?L-1+NAA 0.5 mg?L-1 got the highest rooting rate about 66.70%.And the results of phosphinothricin sensitivity test experiment showed that protein mulberry leaves’ lethality was 50.00%，75.00%，100.00% when concentration of phosphinothricin was 0.04 mg?L-1，0.05 mg?L-1 and 0.07 mg?L-1,respectively.

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Last Update: 2016-10-13