|Table of Contents|

Study on Basidiospore Germination and Biological Characteristics of Primary Hypha in Tricholoma mongolicum(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年16
Page:
136-141
Research Field:
Publishing date:

Info

Title:
Study on Basidiospore Germination and Biological Characteristics of Primary Hypha in Tricholoma mongolicum
Author(s):
LIU Xiaoting; GUO Jiufeng; WANG Shuyan; LI Yajiao; YUAN Lili; NA Ri
(College of Physical Science and Technology/Key Laboratory of Ion Beam Biotechnology，Inner Mongolia University，Huhhot，Inner Mongolia，010021)
Keywords:
Tricholoma mongolicum; growth and development; sexual reproduction; mononuclear mycelia; biological habit
PACS:
-
DOI:
10.11937/bfyy.201616035
Abstract:
Taking Tricholoma mongolicum as materials,the morphology of the basidiospores and mycelium were observed under optical microscope.Situations of basidiospores germination and primary hypha growth in different culture mediums were investigated.The results showed that,basidiospores of Tricholoma mongolicum were kidney-shaped or oval，（4-6） μm×（6-9） μm；it took a week at least when basidiospore could germinate and become macroscopic tiny colony.Adding 1.5 g?L-1yeast extract and 1.5 g?L-1 caseein hydrolysate to the MS medium could promote the basidiospores germination.According to colony growth speed and morphology of primary hypha，we found that there were five distinct types in primary hypha:hypha tomentum type，hypha adhesion type，hyphae thin type，clusters growth type and tardiness growth type.In addition，it was identified which were Tricholoma mongolicum by rDNA-ITS sequence.This result provided a kind of method of PCR directly in mycelium of edible fungus，which simplify the research methods on edible fungus DNA molecules.The results provided the basis for mating type researches and accumulate biological knowledges for the research of edible fungus.

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Last Update: 2016-09-18