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Effect of Root Zone Hypoxia Stress on AsA-GSH Between Two Peony Varieties(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年16
Page:
69-75
Research Field:
Publishing date:

Info

Title:
Effect of Root Zone Hypoxia Stress on AsA-GSH Between Two Peony Varieties
Author(s):
YU Min1; REN Yaping2; MI Yinfa3; CUI Ruihong4
(1.College of Technology Information Business,Zhongyuan University，Zhengzhou，Henan 451191；2.College of Architecture and Urban Planning，Henan University of Urban Construction,Pingdingshan，Henan 467036；3.College of Forestry，Henan University of Science and Technology，Luoyang，Henan 471003；4.School of Foreign Language，Henan University of Science and Technology，Luoyang，Henan 471003)
Keywords:
Paeonia suffruticosa; seedling; hypoxia stress; AsA-GSH cycle system
PACS:
-
DOI:
10.11937/bfyy.201616020
Abstract:
The objectives of this research were to study the effect of hypoxia stress on AsA-GSH system and the mechanism of how free radicals to be scavenged between two different resistance of Paeonia suffruticosa under hypoxia stress.Two 3-year-old strong，uniform peony varieties seedling （P.suffruticosa cv.‘Luoyanghong’and P.suffruticosa cv.‘Huhong’，the former was quite resistant，while the latter was much sensitive to hypoxia according to the preparatory experiments.）were selected and planted in hydroponics.Nutrient solutions were aerated with nitrogen to induce hypoxia stress conditions.Activities of reactive oxygen species (ROS)-scavenging enzymes including ascorbate peroxidase(APX)，glutathione reductase(GR)，contents of antioxidants such as ascorbic acid(AsA),glutathione(GSH)，ratio of total AsA to oxidative ascorbic acid (AsA/DHA)，ratio of GSH to oxidative glutathione (GSH/GSSG) were investigated as well as the contents of H2O2  of leaves and roots.The results showed that，under hypoxia stress，activities of AsA,AsA,DHA,AsA/DHA,GSH,total GSH,GSH/GSSG,APX,GR were all stimulated and increased greatly in leaves and roots.The growth rate and activity peaks of AsA-GSH anti-active oxygen enzymes in leaves and roots of ‘Luoyanghong’ were higher than those of ‘Huhong’.The contents of H2O2 in leaves and roots under hypoxia stress increased significantly.However，the increase ratio and peak contents of H2O2 in leaves and roots of ‘Luoyanghong’ were much lower than those of ‘Huhong’.In conclusion，‘Luoyanghong’ held higher tolerance to root-zone hypoxia stress than ‘Huhong’.

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Last Update: 2016-09-13