|Table of Contents|

Identification，Growth and Polysaccharide Production of a Ganoderma lucidum From Xinjiang(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年15
Page:
142-146
Research Field:
Publishing date:

Info

Title:
Identification，Growth and Polysaccharide Production of a Ganoderma lucidum From Xinjiang
Author(s):
Nuraliya?ABLIMIT1; 2 ; LIN Qing2 ; Zulfiya?YUNUS1
(1.College of Life Sciences and Technology，Xinjiang University，Urumqi，Xinjiang 830046;2.Research Institute of Applied Microbiology,Xinjiang Academy of Agricultural Sciences，Urumqi,Xinjiang 830091)
Keywords:
Xinjiang Ganoderma lucidum; identification; carbon and nitrogen sources
PACS:
-
DOI:
10.11937/bfyy.201615034
Abstract:
Fruiting body of a wild Ganoderma lucidum XJ-001 was obtained from Altay of Xinjiang as the material,with the method of organization separation,the mycelium was isolated and purified from the fruiting body.Genomic DNA of strain XJ-001 was extracted from mycelium，and then used as template for the PCR amplification of ITS fragment.ITS fragment was purified and sequenced.The sequence was analyzed with phylogenetic analysis method.XJ-001 was cultured on five different medias.Optimal mother culture medium was selected by calculating mycelium growth rate.The effect of different carbon and nitrogen sources on mycelium biomass，intracellular and extracellular polysaccharide contents of the strain were studied by shake flask fermentation.The results showed that according to the morphological characteristics combined with rDNA-ITS analysis，the strain XJ-001 was identified as Ganoderma lucidum and GenBank accession number of the ITS was KT997431.The corn soybean agar medium was chosen as excellent mother culture medium,growth rate was 18.7 mm?d-1.The strain XJ-001 was cultured at 30 ℃ and 150 r?min-1 for 5 days using glucose and yeast extract as the carbon and nitrogen source,vespectively.Mycelium biomass，intracellular and extracellular polysaccharide contents were 8.15 mg?mL-1，1.18 mg?mL-1 and 0.05 mg?mL-1，respectively.The results showed that the strain XJ-001 had the potential property to grow rapidly and produce high yield of polysaccharide.The results of this research would provide a reference for the development of Xinjiang wild Ganoderma lucidum resources.

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Last Update: 2016-08-30