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Identification and Biological Characterization of Antagonistic Fungi for Soft Rot Diseases(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年15
Page:
100-104
Research Field:
Publishing date:

Info

Title:
Identification and Biological Characterization of Antagonistic Fungi for Soft Rot Diseases
Author(s):
WEI MiLU LuZHANG WeiFU BenzhongWANG LihuaLI Guoyuan
(Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables/College of Life Science and Technology,Hubei Engineering University,Xiaogan,Hubei 432000)
Keywords:
Penicilium oxalicumidentificationbiological characterizationcamellia diseasesantagonism effect
PACS:
-
DOI:
10.11937/bfyy.201615025
Abstract:
A strain was isolated from soil of cabbage root which could antagonize soft rot diseases through bactericidal test and fermentation culture method.Its anti-bacterium-circle was (37.7±2.2) mm.Detached leaves experiment showed that its control efficienty was 75%.Following morphological characteristics,combining with the result of the rDNA-ITS sequence analysis,it was identified as Phoma herbarum and named Phoma herbarum F217-1.The results indicated that the F217-1 grown well in PSA under 28 ℃,and the lethal temperature was 55 ℃.Although the mycelium could grow between pH 3-10,pH 5-8 were appropriate.Glucose and NaNO3 were the suitable carbon and nitrogen resource,respectively.Ventilation had no significant effect on the growth of penicillium mycelium,but the 24 hours light was not benefit for the mycelia growth.

References:

 

[1]侯明生,黄俊斌.农业植物病理学[M].北京:科学出版社,2014418.

[2]PEROMBELON M.Potato diseases caused by soft rot erwiniasan overview of pathogenesis [J].Plant Pathology200251(1):1-12.

[3]FELIX Kde OLIVEIRA W JMARIANO Ret al.Lettuce genotype resistance to soft rot caused by Pectobacterium carotovorum subsp carotovorum[J].Scientia Agricola201471(4)287-291.

[4]WALERON MWALERON KLOJKOWSKA E.Characterization of Pectobacterium carotovorum subsp odoriferum causing soft rot of stored vegetables[J].European Journal of Plant Pathology2014139(3)457-469.

[5]谷会,朱世江,詹儒林.辣椒采后软腐病拮抗细菌的分离及生防效果[J].热带作物学报,2012(7):1276-1280.

[6]赵江涛,赵延存,黄开红,.芋头软腐病拮抗菌的筛选及生防效果研究[J].食品科学,2014(7):155-159.

[7]马艳,常志州,赵江涛,.一株疫病拮抗青霉 P.st10 菌株的抗菌活性及其对辣椒疫病的盆栽防效[J].中国生物防治,2006,22(3):239-243.

[8]范秀容,沈萍,李广武.微生物学实验[M].北京:高等教育出版社,1999:67-72.

[9]郑琪,徐秉良,薛应钰,.杏采后病害病原拮抗菌的分离筛选及鉴定[J].植物保护,2013,39(4):34-39.

[10]牛慧芹,刘春辉,沈检龙,.玉米大斑病生防细菌的筛选、鉴定及其抑制作用[J].中国农学通报,2014,30(28):275-279.

[11]吴丽媛,刘宝玉,王钰杰,.甜瓜细菌性果斑病生防菌株 BW-6的筛选和鉴定[J].植物保护,2014,40(1):43-47.

[12]傅本重,杨敏,李国元,.滇楸叶斑病病原菌的生物学特性及其抑菌药剂筛选[J].华中农业大学学报,2013(6):65-69.

[13]何月秋.真菌菌丝体培养和提取DNA方法的改进[J].菌物系统,2000,19(3):434.

[14]傅本重,赵文丽,史红安,.吉祥草炭疽病菌生物学特性研究[J].北方园艺,2014(15):116-119.

[15]傅本重,赵文丽,王立华,.吉祥草叶斑病菌的生物学特性及室内防治药剂筛选[J].西南农业学报,2015(1):212-216.

[16]刘君昂,宋光桃,周国英,.一种拮抗油茶病害的菌株及其应用:中国,201110027957.1[P].2011-08-17.

[17]谷祖敏,纪明山,魏松红,.11株草茎点霉培养特征、致病力及RAPD分析[J].吉林农业大学学报,2010(2):140-144.

[18]谷祖敏,纪明山,杨春喜,.草茎点霉SYAU-06菌株与农田常用农药的相容性研究[J].江西农业大学学报,2009(3):504-507.

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Last Update: 2016-08-29