|Table of Contents|

Protein Extraction of Apple Fruit Skin and Two-dimensional Electrophoresis Optimization(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年15
Page:
130-134
Research Field:
Publishing date:

Info

Title:
Protein Extraction of Apple Fruit Skin and Two-dimensional Electrophoresis Optimization
Author(s):
XIAO Long1; 2; CONG Peihua1; 2; ZHANG Caixia1; 2; ZONG Zeran1; 2; TIAN Yi1; 2; ZHANG Liyi1; 2
（1.Key Laboratory of Horticulture Crops Germplasm Resources Utilization，Ministry of Agriculture，Xingcheng，Liaoning 125100；2.Research Institute of Pomology，Chinese Academy of Agricultural Sciences，Xingcheng，Liaoning 125100）
Keywords:
apple; fruit skin; proteomics; two-dimensional electrophoresis (2-DE)
PACS:
-
DOI:
10.11937/bfyy.2016150032
Abstract:
Taking apple fruit skin as test material，and the suitable extraction of apple fruit skin protein was selected，and the two-dimensional electrophoresis (2-DE) operating steps of the protein sample were improved.The results showed that the modified phenol extraction was suited for the extraction of apple fruit skin protein，and the improved 2-DE operating system (IPG strip 18 cm pH 4-7，600 μg sample loading)，the improved program of isoelectric focusing，16% SDS-PAGE gel was suited for the separation of the protein.

References:

 

[1]PANDEY A，MANN M，Proteomics to study genes and genomics[J].Nature，2000，405(6788)：837-846.

[2]杨礼富.蛋白质组学研究技术与展望[J].热带农业科学，2007,27(2)：58-62.

[3]QIN G Z，TIAN S P，CHAN Z L，et al.Crucial role of antioxidant proteins and hydrolytic enzymes in pathogenicity of Penicillium expansum：analysis based on proteomic approach[J].Mol Cell Proteomics，2007（6）：425-438.

[4]CHANG W，HUANG L，SHEN M，et al.Patterns of protein synthesis and tolerance of anoxia in root tips of maize seedlings acclimated to a low-oxygen environment，and identification of proteins by mass spectrometry[J].Plant Physiol，2000，122：295-318.

[5]王清，产祝龙，秦国政，等.果实蛋白质组学研究的实验方法[J].植物学报，2009,44(1):107-116.

[6]GIRIBALDI M,PERUGINI I,SAUVAGE F X，et al.Analysis of protein changes during grape berry ripening by 2-DE and MALDI-TOF[J].Proteomics，2007，7(17):3154-3170.

[7]VELASCO R，ZHARKIKH A，AFFOURTIT J，et al.The genome of the domesticated apple(Malus×domestica Borkh.)[J].Nature Genetics，2010，42:833-839.

[8]曹尚银，张秋明，朱志勇，等.苹果花芽孕育蛋白质组学初步分析[J].中国农业科学，2007,40(10):2281-2288.

[9]CAO X，GAO Y，WANG Y，et al.Different expression and modification of protein during ontogenesis in Malus domestica[J].Proteomics，2011，11(24):4688-4701.

[10]张彩霞，田义，张利义，等.苹果枝条表皮应答轮纹病菌侵染的蛋白质组学分析[J].植物病理学报,2015,45(3)：280-287.

[11]SONG J，BRAUN G，BEVIS E，et al.A simple protocol for protein extraction of recalcitrant fruit tissues suitable for 2-DE and MS analysis[J].Electrophoresis，2006，27(15):3144-3151.

[12]CHAN Z L，QIN G Z，XU X B，et al.Proteome approach to characterize proteins induced by antagonist yeast and salicylic acid in peach fruit[J].Journal of Proteome Research，2007，6(5)：1677-1688.

[13]ISAACSON T，DAMASCENO C M B，SARAVANAN R S，et al.Sample extraction techniques for enhanced proteomic analysis of plant tissues[J].Nature Protocols，2006，1(2):769-774.

[14]WANG X Q，YANG P F，GAO Q，et al.Proteomic analysis of the response to high-salinity stress[J].Planta，2008，228:167-177.

[15]ROBERT W，ALOIS H，ANGEL G，et al.Towards higher resolution：Two-dimensional electrophoresis of Saccharomyces cerecisiae proteins using overlapping narrow immobilized pH gradients[J].Electrophoresis，2000，21:2610-2616.

[16]丁承强，马丹，王绍华，等.水稻蛋白质组双向电泳优化流程及方法[J].植物学报，2011,46(1):67-73.

Memo

Memo:
-
Last Update: 2016-08-29