|Table of Contents|

Effect of 6-Benzylaminopurine on Growth and Antioxidase Activities in Hairy Root Cultures of Pueraria phaseoloides(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年14
Page:
156-159
Research Field:
Publishing date:

Info

Title:
Effect of 6-Benzylaminopurine on Growth and Antioxidase Activities in Hairy Root Cultures of Pueraria phaseoloides
Author(s):
HE Hanjie1; HUANG Xiaoxi1; ZHANG Dangquan1; DENG Huafeng2
（1.Hunan Provincial Key Laboratory of Forestry Biotechnology，Central South University of Forestry and Technology/Cooperative Innovation Center of Engineering and New Products for Developmental Biology，Changsha，Hunan 410018;2.Hunan Hybrid Rice Research Centre，State Key Laboratory of Hybrid Rice，Changsha，Hunan 410125）
Keywords:
Pueraria phaseoloides; hairy roots; 6-Benzylaminopurine; isoflavone compounds; soluble protein; antioxidase
PACS:
-
DOI:
10.11937/bfyy.201614039
Abstract:
Using the hairy roots of Pueraria phaseoloides cultured in solid mediums as an experiment material，the effect of 6-Benzylaminopurine (6-BA) on growth，contents of isoflavone compounds and soluble protein and antioxidase activities was studied.The results showed that plant growth regulator 6-BA inhibited the growth of P.phaseoloides hairy roots，reduced the biomass of fresh weight of hairy roots compared with control，and the inhibition was in proportion to the concentration of 6-BA.Furthermore，6-BA promoted synthesis and accumulation of isoflavone compounds in hairy root cultures.Compared with control，6-BA decreased the contents of soluble protein and activities of superoxide dismutase in hairy root cultures，but enhanced the activities of peroxidase.These results indicated that plant growth regulator 6-BA regulated the growth and accumulation of isoflavone compounds through regulating antioxidase activities of P.phaseoloides hairy roots.

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Last Update: 2016-08-17