|Table of Contents|

Extraction and Column Chromatography Purification Technology of Xanthoceraside and Antimicrobial Activities of Different Fractions(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年14
Page:
144-147
Research Field:
Publishing date:

Info

Title:
Extraction and Column Chromatography Purification Technology of Xanthoceraside and Antimicrobial Activities of Different Fractions
Author(s):
LI Jinhuan1YU Jinghua2WANG Lihua2YUAN Shusheng1YIN Liming2WANG Lili1
(1.Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin,Heilongjiang 150040;2.Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang,Liaoning 110016)
Keywords:
xanthocerasideextractionsilica gelseparation and purificationanti-microbial activity
PACS:
-
DOI:
10.11937/bfyy.201614036
Abstract:
Taking Xanthoceras sorbifolia Bunge.shell as material,effects of materials liquid ratio (ML),ultrasound temperature (UTe),ultrasound time (UTi) and ultrasound intensity (UI) on extraction rate of crude extract were studied and the optimize extraction conditions were done by orthogonal experiment.Then the xanthoceraside was separated and purified through silica gel H and ODS-A column.Finally,to research the anti-microbial activities of different fractions from purifications,three kinds of bacteria and two kinds of fungi were collected.The results showed that the optimize extraction conditions were that the UTe,UI,UTi,SR was 70 ℃,180 W,30 minutes,1∶25 g?mL-1,respectively;the optimize extraction rate was 17.26%.The content and purity of xanthoceraside was the highest in the 80% methanol-water fraction,which anti-microbial activities were presented on Staphylococcus aureus and Candida albicans. Some theoretical basis were provided for the optimization of the extraction and purification techniques and the research of anti-microbial activities of xanthoceraside.

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Last Update: 2016-08-17