|Table of Contents|

Rapid Detection of Tomato Yellow Leaf Curl Virus Resistant Gene ty-5 in Tomato by PCR(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年14
Page:
110-114
Research Field:
Publishing date:

Info

Title:
Rapid Detection of Tomato Yellow Leaf Curl Virus Resistant Gene ty-5 in Tomato by PCR
Author(s):
YANG Huanhuan1JIANG Jingbin1LI Jingfu1XU Xiangyang1SHANG Junming2
(1.College of Horticulture,Northeast Agricultural University,Harbin,Heilongjiang 150030;2.College of Literature and Media,Taishan University,Tai’an,Shandong 271000)
Keywords:
tomato yellow leaf curl virusty-5SSRPCR
PACS:
-
DOI:
10.11937/bfyy.201614027
Abstract:
Taking CLN32120a-23(resistant materials)and Moneymaker(susceptible materials)as experimental materials which is resistant to yellow leaf curl virus in tomato,F1、F2 were obtained through hybridization,selfcross,using SSR technology,a molecular marker was obtained.The results showed that SSR marker had a 550 bp amplification fragment in homozygous resistant lines,a 780 bp fragment in susceptible lines and both 780 bp and 550 bp fragments in heterozygous resistant lines.The detection results were consistent with those of field identification.So the SSR marker,tightly linked to ty-5 gene,could distinguish homozygous and heterozygous resistant lines and susceptible lines.27 tomato materials individuals were detected.There were 15 homozygous resistant lines,the molecular detection was 80% coincident with the results of field identification.The results showed that ty-5 SSR marker could be used for rapid identification of ty-5 resistant gene in tomato.

References:

 

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Last Update: 2016-08-17