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Construction of Recombinant Vector of AmAS 1 and Am4’CGT Genes and the Transient Expression in Malus Flowers(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年14
Page:
97-102
Research Field:
Publishing date:

Info

Title:
Construction of Recombinant Vector of AmAS 1 and Am4’CGT Genes and the Transient Expression in Malus Flowers
Author(s):
LIU Xiaowei1; LI Houhua1; WANG Bingjie2; FEI Zhaoxue2; QUE Yi3
(1.College of Landscape Architecture and Arts，Northwest Agriculture and Forestry University，Yangling,Shaanxi 712100；2.College of Forestry，Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100;3.City College of Science and Technology,Chongqing University，Chongqing 402167)
Keywords:
crabapple; aurones; bivalent expression vector; transient expression
PACS:
-
DOI:
10.11937/bfyy.201614024
Abstract:
Taking Antirrhinum majus and Malus ‘Snow drift’ as materials，using agrobacterium-mediated method，the recombinant vector containing Am4’CGT and AmAS1 was expressed in Malus flowers，the instantaneous expression effect was identified by HPLC and PCR analysis.The results showed that the recombinant vector CGT-AS-pCambia 1302 was built successfully，after infected by EHA105，the Malus flowers appeared to be light yellow，and it indicated that the target genes were transcribed and expressed in Malus flowers.A new absorption peak was found by HPLC analysis，the result indicated that there were aurones accumulated.

References:

 

[1]李厚华，赵冰，李鹏民.中国海棠[M].北京:中国林业出版社，2014:1-13.

[2]高立旦，童再康，曹件生，等.彩叶木本植物育种研究进展[J].浙江林业科技，2006，26(4)：77-82.

[3]TANAKA Y，SASAKI N，OHMIYA A.Biosynthesis of plant pigments：anthocyanins，betalains and carotenoids[J].The Plant Journal，2008,54：733-749.

[4]周琳，王雁，彭镇华.黄色花形成机制及基因工程研究进展[J].林业科学，2009，45(2)：111-119.

[5]TANAKA Y，ONO E，NAKAMURA N，et al.Method for producing yellow flower by controlling flavonoid synthetic pathway:US 7750209 B2[P].U.S.Patent，International Flower Developments Proprietary Limited.

[6]NAKAYAMA E.Enzymology of aurone biosynthesis[J].Journal of Bioscience and Bioengineering,2002,94(6)：487-491.

[7]NAKAYAMA T，SATO T，FUKUI Y，et al.Specificity analysis and mechanism of aurone synthesis catalyzed by aureusidin synthase，a polyphenol oxidase homolog responsible for flower coloration[J].FEBS Letters，2001，499(1-2)：107-111.

[8]ONO E，FUKUCHI-MIZUTANI M，NAKAMURA N，et al.Yellow flowers generated by expression of the aurone biosynthetic pathway[J].Proceedings of the National Academy of Sciences of the United States of America，2006，103(29)：11075-11080.

[9]KAPILA J，RYCKE R D，MONTAGU M V，et al.An agrobacterium mediated transient gene expression system intact leaves[J].Plant Science，1997,122：101-108.

[10]ZHANG Y Z，LI P M，CHENG L L.Developmental changes of carbohydrates，organic acids，amino acids，and phenolic compounds in ‘Honeycrisp’ apple flesh[J].Food Chemistry，2010,123：1013-1018.

[11]宋建，刘仲齐.农杆菌介导的基因瞬时表达技术及其应用[J].天津农业科学，2008,14(1)：20-22.

[12]邱礽，陶刚，李奇科，等.农杆菌渗入法介导的基因瞬时表达技术及应用[J].分子植物育种,2009，7(5)：1032-1039.

[13]NAKAYAMA T，YONEKURA-SAKAKIBARA K，SATO T，et al.Aureusidin synthase：A polyphenol oxidase homolog responsible for flower coloration[J].Science，2000,290(5494)：1163-1166.

[14]OREN S M，LEVI N A.Temperature effect on leaf pigmentation of Cotinus coggygri ‘Royal Purple’[J].Journal of Horticulture Science，1997,72：425-432.

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Last Update: 2016-08-16