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Extraction of Atrazine-degrading Enzymes and Enzyme Activity Analysis(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年14
Page:
94-96
Research Field:
Publishing date:

Info

Title:
Extraction of Atrazine-degrading Enzymes and Enzyme Activity Analysis
Author(s):
GAO Guifeng; WANG Junling; CHU Haijiao
（School of Bio-engineering，Jilin Agricultural Science and Technology University，Jilin，Jilin 132101）
Keywords:
atrazine-degrading enzymes; HPLC; degradability
PACS:
-
DOI:
10.11937/bfyy.201614023
Abstract:
Taking atrazine degradation strains as experimental material.Crude enzyme was extracted by the ultrasonic wall-breaking water methods.Residual atrazine was extracted with chloroform in the culture medium.The residues of atrazine was measured by HPLC.Under different conditions of temperature，pH，crude enzyme fluid content，training time，crude enzyme was studied on the influence of atrazine degradation rate,in order to make better use of atrazine-degrading enzymes.The results showed that atrazine degradation enzyme of optimum temperature was about 35 ℃.The optimal pH was 7.5.When the crude enzyme liquid content was above 50%，the substrate degradation was complete.

References:

 

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Last Update: 2016-08-16