Taking jujube fruit as material,adopting the homologous cloning method,based on transcriptome sequencing results,the jujube fruit F3H full-length gene was cloned.The bio-information analysis and the F3H influence on anthocyanins were studied.The results showed that the F3H gene was 1 336 bp,encoding 367 amino acid residues.According to the results of gene sequence alignment,sequence of amino acids of F3H gene had the consistency of 90%,90%,89% and 90% compared with Dimocarpus longan,Canarium album,Litchi chinensi and Morus notabilis,respectively.Semi-quantitative RT-PCR analysis showed that the gene expression in jujube fruit red ripe period was the most,followed by a half red jujube fruit,and jujube fruit green ripe stage expression quantity was minimum；extracting anthocyanins in jujube during different periods,the change of anthocyanins content in jujube,was consistent with gene expression quantity.