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Study on Enzymatic-ultrasonic Assisted Extraction of Polysaccharide From Sophora japonica Linn. and Its Free Radical Scavenging Activity(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年13
Page:
141-146
Research Field:
Publishing date:

Info

Title:
Study on Enzymatic-ultrasonic Assisted Extraction of Polysaccharide From Sophora japonica Linn. and Its Free Radical Scavenging Activity
Author(s):
CAO Xiaoyan; YANG Haitao
(School of Chemical and Environmental Science,Shaanxi University of Technology,Hanzhong,Shaanxi 723001)
Keywords:
Sophora japonica Linn. polysaccharide; orthogonal experiment; cellulase; citrate buffer solution
PACS:
-
DOI:
10.11937/bfyy.201613037
Abstract:
Taking Sophora japonica Linn. from Qinba area as raw material,citrate buffer solution at pH 6.0 as the extraction solvent,based on single factor experiments and orthogonal test,the extraction and scavenging free radicals activities of polysaccharides from Sophora japonica Linn. by cellulase-ultrasonic were studied.The results showed that the optimum extraction technology of Sophora japonica Linn. polysaccharides were as follows,extraction temperature was 60 ℃,ultrasonic extraction time of 20 minutes,solid-liquid ratio of 1∶25 kg?L-1,cellulase dosage was 10 mg?g-1,under this condition,the extraction yield of polysaccharide was 17.1%.Scavenging free radicals activities results showed that the IC50 of ABTS+,?OH,O?[TX-*4]2 were 0.047 g?L-1,0.75 g?L-1 and 0.87 g?L-1 respectively,which was superior to the conventional antioxidants of ascorbic acid.The study proved that polysaccharides from Sophora japonica Linn. had good reducing capacity and scavenging radical activity. Keywords:Sophora japonica Linn. polysaccharide；orthogonal experiment；cellulase；citrate buffer solution

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Last Update: 2016-08-11