|Table of Contents|

Tissue Culture and Rapid Propagation of Lonicera ruprechtiana Regel(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年13
Page:
107-110
Research Field:
Publishing date:

Info

Title:
Tissue Culture and Rapid Propagation of Lonicera ruprechtiana Regel
Author(s):
WANG Huan12XU Shizhao3LI Xu1
(1.College of Forestry,Beihua University,Jilin,Jilin 132013;2.Jilin Province Key Laboratory of Forestry and Ecological Environment,Jilin,Jilin 132013;3.Forest Survey and Design Institute of Jilin Province,Changchun,Jilin 130022)
Keywords:
Lonicera ruprechtiana Regelmicropropagation in vitrosegments of tender stems
PACS:
-
DOI:
10.11937/bfyy.201613028
Abstract:
Taking tender stem segments of Lonicera ruprechtiana Regel as explants,MS was selected as the basic medium supplemented with different plant growth regulators,the rapid propagation was studied.The results showed that the surface sterilization in 0.1% HgCl2 solution adding 0.1% Tween 80 for 5 min was the best.The optimal medium for inducing axillary buds was MS+1.0 mg?L-1 6-BA+0.05 mg?L-1 NAA+30 g?L-1 sucrose+7 g?L-1 agar or MS+30 g?L-1 sucrose+7 g?L-1 agar;the medium MS+1.0 mg?L-1 6-BA+0.05 mg?L-1 NAA+30 g?L-1 sucrose+7 g?L-1 agar was good for multiplication and the proliferation coefficient was 8.The optimum medium for root induction was 1/2MS+15 g?L-1 sucrose+7 g?L-1 agar,and the rooting rate was 60.0%.

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Last Update: 2016-08-10