|Table of Contents|

Cloning and Bioinformatical Analysis of DAHPS Gene in Pomegranate Peel(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年13
Page:
98-103
Research Field:
Publishing date:

Info

Title:
Cloning and Bioinformatical Analysis of DAHPS Gene in Pomegranate Peel
Author(s):
FENG Lijuan1; YIN Yanlei1; YANG Xuemei1; WU Chong1; LI Yingpeng2
(1.Shandong Institute of Pomology,Tai’an,Shandong 271000；2.Dianzi Forestry Station of Guan County in Shandong Province,Liaocheng,Shandong 252525)
Keywords:
pomegranate; DAHPS gene; cloning; sequence analysis; gallic acid
PACS:
-
DOI:
10.11937/bfyy.201613026
Abstract:
Taking ‘Taishanhong’ pomegranate as test material,the DAHPS gene was cloned by using RT-PCR technology and the bioinformatical characteristics were analyzed.The results showed that the cDNA was 1 373 bp,and it encoded 393 amino acids.The similarities of pomegranate DAHPS gene compared with Citrus sinensis (XP_006472576.1),Pyrus bretschneideri (XP_009372963.1),Vitis vinifera(NP_001268096.1),Malus domestica (XP_008386115.1)were 94%,93%,92% and 92%,respectively.The theoretical molecular mass of DAHPS protein was 44 kDa,and isoelectric point was 9.00.It was hydrophilic and transmembrane protein,but not secreted,which had Cys-Ser-Ala-His and typical DAHP_synthe_2 superfamily conserved domain.The secondary structure of DAHPS protein was composed by random coil(40.97%),α-helix(38.17%),extended strand(12.47%)and β-turn(8.40%).The GenBank accession number was KU198932.The result established a theoretical foundation for revealing the metabolic mechanism of gallic acid in pomegranate fruit.

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Last Update: 2016-07-29