|Table of Contents|

Analysis of Characteristic of Codon Usage of Eucommia ulmoides Transcriptome(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年13
Page:
85-89
Research Field:
Publishing date:

Info

Title:
Analysis of Characteristic of Codon Usage of Eucommia ulmoides Transcriptome
Author(s):
LIU Huimin12WUYUN Tana12DU Hongyan12
(1.Non-timber Research and Development Center,Chinese Academy of Forestry Science,Zhengzhou,Henan 450003;2.The Eucommia Engineering Research Center of State Forestry Administration,Zhengzhou,Henan 450003)
Keywords:
Eucommia ulmoidestranscriptomecodonbias
PACS:
-
DOI:
10.11937/bfyy.201613023
Abstract:
Eucommia ulmoides was used as test materials,the correlation between codon composition and gene expression was analyzed based on the transcriptome data by using CodonW and SPSS software,in order to offer a theoretical foundation for transforming exogenous gene and Eucommia genetic engineering.The results showed that the gene expression had a significant and positive correlation to G3s,C3s,G+C and GC3s(P<0.01)content and a negative correlation to A3s content,T3s content,gene coding sequence length and effective codon number(ENC)(P<0.01)and Aro content(P<0.05).These results indicated that genes with a high expression had a stronger codon usage bias and likely end with G/C.ENC had a significant positive correlation to A3s,T3s and Aro content(P<0.01)and a significant negative correlation to C3s,G3s,G+C,and GC3s content(P<0.01).Fifteen optimal codons were identified,namely,AAC,AAG,ACC,AGG,AUC,CAC,CAG,CCG,CUC,CUG,GCC,GGC,GUC,UAC,and UCC.Fruit and leaf special genes had difference on the codon usage of Leu,Ile,Ser,Pro,Thr,Asn,Glu and Cys.

References:

 

[1]ZHANG G Y,GE H H,FANG B S.Analysis of synonymous codon usage in teo hyperthermophiles[J].Journal of Huaqiao University,2006,22(2):87-90.

[2]CARLINI D B,STEPHAN W.In vivo introduction of unpreferred synonymous codons into the Drosophila Adh gene results in reduced levels of ADH protein[J].Genetics,2003,163:239-243.

[3]SHARP P M,MATASSI G.Codon usage and genome evolution[J].Curr Opin Genet Dev,1994(4):851-860.

[4]LIU Q P,FENG Y,ZHAO X A,et al.Synonymous codon usage bias in oryza sativa[J].Plant Science,2004,67:101-105.

[5]ROMERO H,MUSTO H,ZAVALA A.Codon usage in Chlamydia trachomatis is the result of strand-specific mutational biases and a complex pattern of selective forces[J].Nucleic Acids Res,2000,28(10):2084-2090.

[6]STENICO M,LLOYD A T,SHARP P M.Codon usage in Caenorhabditis elegans:delineation of translational selection and mutational biases[J].Nucl Acid Re,1994,22:2437-2246.

[7]OLEJNICZAK M,UHLENBECK O C.tRNA residues that have coevolved with their anticodon to ensure uniform and accurate codon recognition[J].Biochinmie,2006,88:943-950.

[8]张文娟.基于密码子水平的生物信息学分析及进化研究[D].上海:复旦大学,2006.

[9]ROMERO H,ZAVALA A,MUSTO H,et al.The influence of translational selection on codon usage in fishes from the family Cyprinidae[J].Gebe,2003,317:141-147.

[10]HIRAOKA Y,KAWAMATA [KG-*4]K,HARAGUCHI T,et al.Codon usage bias is correlated with gene expression levels in the fission yeast Schizosaccharomyces pombe[J].Genes Cells,2009,14(4):499-509.

[11]孙铮,马亮,MURPHY R,.Wolbachia pepientis wMEL基因组水平上的密码子使用分析[J].中国科学C(生命科学),2009,39(10):948-953.

[12]KNIGHT R D,FREELAND S J,LANDWEBER L F.A simple model based on mutation and selection explains rends in codon and amino-acid usage and GC composition within and across genomes[J].Genome Biol,2001(2):RESERCH0010.

[13]GU W J,ZHOU T,MA J M,et al.Folding type specific secondary structure propensities of synonymous codons[J].IEEE Trans Nanobiosci,2003,2(3):150-157.

[14]杜红岩.杜仲活性成分与药理研究的新进展[J].经济林研究,2003,21(2):58-61.

[15]杜红岩.我国的杜仲胶资源及其开发潜力与产业发展思路[J].经济林研究,2009,28(3):1-5.

[16]曹慧颖,张锐,郭三堆.串联的人胸腺素a1基因在番茄中的高效表达[J].中国农业科学,2009,42(7):2291-2296.

[17]邹永梅,施季森,诸葛强,.遗传转化植物中沉默基因的消除[J].分子植物育种,2004,4(1):95-102.

[18]SHARP P M,HANEY T M F,MOSURSKI K R.Codon usage in yeast:cluster analysis clearly differentiates highly and lowly expressed genes[J].Nucl Acids Res,1986(14):5125-5143.

[19]SAU K,GUPTA S K,SAU S,et al.Analysis of factors shaping codon usage in the mitochondrion genome of Oryza sativa[J].Mitochondrion,2004(4):313-320.

[20]WRIGHT F.The effective number of codons used in a gene[J].Gene,1990,87:23-29.

[21]GUPTA S K,BHATTACHARYYA T K,GHOSH T C.Synonymous codon usage in lactococcus latis:mutational bias versus translational selection[J].Biomol Struct Dyn,2004(21):1-9.

[22]DONALD S,HAGEN T,FABIAN G,et al.A single-molecular long-read survey of the human transcriptome[J].Nature Biotechnology,2013(31):1009-1014.

[23]HAGEN T,FABIAN G,DONALD S,et al.Defining a personal,allele-specific,and single molecule long-read transcriptome[J].PNAS,2014,111:9869-9874.

Memo

Memo:
-
Last Update: 2016-07-28