Study of Tolerance to Low Temperature in Viola philippica, an Early Spring Cover Plant(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study of Tolerance to Low Temperature in Viola philippica, an Early Spring Cover Plant

Author(s):: CHANG Libo; JIA Lingyun; SU Xue; QIN Jingjing; SUN Kun; (College of Life Science,Northwest Normal University,Lanzhou,Gansu 730070)

Keywords:: Viola philippica tissue cultured seedling; low temperature; physiological changes; tolerance

Abstract:: The physiological changes of Viola philippica(an early spring covered plant)were detected to study its tolerance to low temperature environment.The results showed that with the increase of treatment time,the content of MDA increased at first and decreased subsequently going stable finally at 16 ℃,10 ℃ and 4 ℃,respectively.However,the activity of SOD showed the opposite trend,indicating that the tissue cultured seedling of V.philippica had capacity to improve the harmfulness of cold environment although the membrane system was injured to some extent.The chlorophyll content of tissue cultured seedlings was affected at different temperatures especially at 4 ℃,but could be recovered to some extent.Under the treatment low temperature at 16 ℃,10 ℃ and 4 ℃,respectively,Fo showed no significant change compared with different temperature treatment while Fv/Fm decreased.With the extension of treatment time,the increasing of Fv/Fm indicated that low temperature had a reversible harm to PSII reaction center.The qP value firstly decreased then recovered to some extent and qN value increased indicating that V.philippica improved the capacity of receiving electron and its photosynthetic system of heat dissipation to improve the injury of low temperature.All of the physiological changes under low temperature(4~16 ℃)implied the tissue cultured seedlings of V.philippica had tolerance to low temperature.

[1]潘瑞炽,董愚德.植物生理学[M].北京:高等教育出版社,1995.

[2]陈启洁,吴姝菊,李君霞,等.紫花地丁的开发利用与栽培技术研究[J].国土与自然资源研究,2004(1):95-96.

[3]石爱平,王红利.紫花地丁的几种抗逆指标研究初探[J].北京农学院学报,1997,12(1):48-53.

[4]付印东,孙晓梅,杨宏光,等.紫花地丁组培快繁技术研究[J].北方园艺,2008(5):200-201.

[5]王学奎.植物生理生化实验原理和技术[M].北京:高等教育出版社,2006.

[6]郝建军,康宗利,于洋.植物生理学实验技术[M].北京:化学工业出版社,2006.

[7]陈建明,俞晓平,程家安.叶绿素荧光动力学及其在植物抗逆生理研究中的应用[J].浙江农业学报,2006,18(1):51-55.

[8]计淑霞,戴绍军,刘炜.植物应答低温胁迫机制的研究进展[J].生命科学,2010(10):1013-1019.

[9]陈亚华,沈振国.低温,高pH胁迫对水稻幼苗根系质膜,液泡膜ATP酶活性的影响[J].植物生理学报,2000,26(5):407-412.

[10]张学财,周文钊,李俊峰.低温胁迫下4个剑麻品种的SOD,POD,CAT变化[J].中国热带农业,2009(4):47-50.

[11]马博英,金松恒,徐礼根,等.低温对三种暖季型草坪草叶绿素荧光特性的影响[J].中国草地学报,2006,28(1):58-62.

[12]田长恩,梁承邺.多胺对水稻CMS系及其保持系幼穗蛋白质,核酸和活性氧代谢的影响[J].植物生理学报,1999,25(3):222-228.

[13]刘慧英,朱祝军,吕国华.低温胁迫对嫁接西瓜耐冷性和活性氧清除系统的影响[J].应用生态学报,2004,15(4):659-662.

[14]薛立,任向荣,曹鹤,等.低温胁迫对六种苗木生理特性的影响[J].生态学杂志,2008,27(4):524-531.

Last Update: 2016-07-28