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Cloning and Expression of cDNA Encoding Key Enzymes(Isopentenyl Diphosphate  Isomerase)in Diterpenoids Biosynthesis Pathway From  Rabdosia rubescens Henrsl.Hara(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年12
Page:
80-85
Research Field:
Publishing date:

Info

Title:
Cloning and Expression of cDNA Encoding Key Enzymes(Isopentenyl Diphosphate  Isomerase)in Diterpenoids Biosynthesis Pathway From  Rabdosia rubescens Henrsl.Hara
Author(s):
SU Xiuhong; YIN Lei; CHEN Suiqing
(College of Pharmacy,Henan University of Traditional Chinese Medicine,Zhengzhou,Henan 450046)
Keywords:
Rabdosia rubescens Henrsl.Hara; diterpenoid; IDI
PACS:
-
DOI:
10.11937/bfyy.201612021
Abstract:
cDNAs encoding isopentenyl diphosphate isomerase were obtained from the leaves of aseptic seedlings of Rabdosia rubescens by reverse transcription PCR based on the results of transcriptome sequencing.The results showed that the full-length cDNA of IDI was 1 050 bp and contained an open reading frame(ORF)of 906 bp,encoding 301 amino acids.The theoretical molecular weight was 27.4 kDa and the isoelectric point was predicted as 5.06 and it was a kind of hydrophilic protein.Real-time quantitative PCR was used to detect the relative expression levels of of IDI in different tissues of Rabdosia rubescens.And the expression of IDI was relatively high in the leaves,the lowest expression in callus.

References:

 

[1]马靓,丁鹏,杨广笑,等.植物萜类生物合成途径及关键酶的研究进展[J].生物技术通报,2006(增刊):22-30.

[2]CAMPBELL M,HAHN F M,POULTER C D,et al.Analysis of the isopentenyl diphosphate isomerase gene family from Arabidopsis thaliana[J].Plant Molecular Biology,1998,36(2):323-328.

〖LL〗

[3]LIAO Z H,CHEN M I,YANG Y J.A new isopentenyl diphosphate isomerase gene from sweet potato:cloning,characterization and color complementation[J].Biologia,2008,63(2):221-226.

[4]王伟,陆辉强,刘万宏.玉米异戊烯基焦磷酸异构酶基因的克隆及其功能分析[J].西北植物学报,2008,28(9):1715-1719.

[5]仇键,刘实忠,张志平,等.橡胶草异戊烯焦磷酸异构酶基因的电子克隆及分析[J].生物信息学,2013,11(3):209-215.

[6]CHEN R,HARADA Y,TAKESHI B,et al.Overexpression of an isopentenyl diphosphate isomerase gene to enhance trans -polyis oprene production in Eucommia ulmoides Oliver[J].BMC Biotechnology,2012(12):387-390.

[7]张伟,胡晓清,王小元.阪崎克罗诺杆菌中异戊二烯焦磷酸异构酶(IDI)增强类胡萝卜素合成的研究[J].工业微生物,2014,44(5):23-29.

[8]苏秀红,李雪菊,董诚明,等.冬凌草甲素、迷迭香酸在冬凌草植株内空间积累的动态规律[J].中国医药导报,2009,6(21):5-6.

[9]张艳贞,董诚明,苏秀红,等.稀土元素对冬凌草愈伤组织生长及冬凌草甲素、乙素含量的影响[J].中国实验方剂学杂志,2013,19(3):132-135.

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Last Update: 2016-07-21