|Table of Contents|

The Initial Research on Approach of Quickly Capture Meiosis Period and Observation of Meiosis Process in Pollen Mother Cell in Paeonia ostii(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年11
Page:
100-105
Research Field:
Publishing date:

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Title:
The Initial Research on Approach of Quickly Capture Meiosis Period and Observation of Meiosis Process in Pollen Mother Cell in Paeonia ostii
Author(s):
ZHANG Linjuan1; YUAN Junhui2; 3; YU Shuiyan2; 3; HU Yonghong2; 3
(1.Shanghai Normal University，Shanghai 200234；2.Shanghai Chenshan Plant Science Research Center，the Chienese Academy of Science，Shanghai Chenshan Botanical Garden，Shanghai 201602；3.Shanghai Key Laboratory of Plant Functional Genomics and Resources，Shanghai Chenshan Botanical Garden，Shanghai 201602)
Keywords:
Paeonia ostii; chromosome; meiosis; microspore; chromosome bridge
PACS:
-
DOI:
10.11937/bfyy.201611026
Abstract:
The Paeonia ostii buds was taken as the material，then the effects of flower bud size，anther color and average temperature on pollen mother cell meiosis were researched by consecutive observation of sampling and slide-preparing technique of chromosome.And the detailed cell chromosomes morphological changes in the whole process of pollen mother cell meiosis and the process of pollen grain formation of Paeonia ostii was characterized in this paper.The results showed that the temperature，bud size，and anther color were the key factors which influence the meiosis through based on consecutive observation of sampling，the best observation period of meiosis in tree peony when the daily average temperature was 6.5-15.5 ℃,the bud diameter size was 12-16 mm，and the anther was bright yellow.The formation of pollen grain in P.ostii mainly consist of pollen mother cell meiosis stage，metaphase，early stage of mononuclear microspore，later stage of momonuclear microspore and two nuclear microspore stage and then form pollen grain finally.The research observed P.ostii meiosis exist anomalies further，such as chromosome bridge，abnormal tetrad.This research provided basic technical support for obtaining the single chromosome of tree peony，further lay a foundation for molecular biology research and genetic breeding in chromosome level，and provided the reference for other plants.

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Last Update: 2016-07-14