|Table of Contents|

Induction of Two Genotypes Cucumber Callus(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年11
Page:
96-99
Research Field:
Publishing date:

Info

Title:
Induction of Two Genotypes Cucumber Callus
Author(s):
DU YanlingZHAO XintaoSU Feng
(School of Chemical Engineering,Qingdao University of Technology,Qingdao,Shandong 266042)
Keywords:
cucumbercotyledonhypocotylcallustissue culture
PACS:
-
DOI:
10.11937/bfyy.201611025
Abstract:
Two different kinds of cucumbers,‘Jingu six’ and ‘Gillette 168’ were selected as the materials,cotyledon and hypocotyl as explants,the effect of culture medium type,hormone concentration,hormone ratio on callus induction and differentiation of adventitious bud were investigated.The results showed that both cucumbers showed the strong callus differentiation ability,and hypocotyl was superior to cotyledon;the shape of the callus was quite different under different hormone ratios.AgNO3 as the additives could commendably restrain the vitrification of the callus induction;both cucumbers had the same best hormone ratio for cotyledon explant MS+0.5 mg?L-1 6-BA+0.10 mg?L-1 IAA+1.0 mg?L-1 AgNO3 and for hypocotyl explant MS+2.0 mg?L-1 6-BA+0.10 mg?L-1 IAA+1.0 mg?L-1 AgNO3.The differentiation of adventitious bud was not formed.

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Last Update: 2016-07-14