|Table of Contents|

Cloning and Expression of Faapx-c Gene and Bioinformatics Analysis in Fragaria×ananassa Based on RACE(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年11
Page:
88-92
Research Field:
Publishing date:

Info

Title:
Cloning and Expression of Faapx-c Gene and Bioinformatics Analysis in Fragaria×ananassa Based on RACE
Author(s):
HOU Yanxia1; TANG Haoru2; LIN Yuanxiu2; CHEN Qing2; ZHANG Yong2; GUO Yan1
（1.Department of Horticulture，Shanxi Forestry Vocational Technical College，Taiyuan，Shanxi 030009；2.Horticultural College,Sichuan Agricultural University，Ya’an，Sichuan 625014）
Keywords:
Fragaria×ananassa; Faapx-c gene; RACE; cloning; expression; bioinformatics
PACS:
-
DOI:
10.11937/bfyy.201611023
Abstract:
Taking Fragaria×ananassa cv.Toyonaka as material，a cytosolic ascorbate peroxidase gene Faapx-c was cloned by RT-PCR and 3′-RACE technology，and the bioinformatic analysis of Faapx-c was carried out.The results showed that Faapx-c was 1 034 bp in length coding a protein FaAPX-c，which was a stable，hydrophilicity and non-secreted protein.The secondary structure of FaAPX-c contained mainly α-helix and random coil，with reliable 3D structure.And the protein belonging to a member of plant peroxidase superfamily.Expression analysis of Faapx-c gene showed that it was induced by low temperature in roots，stems，leafs，and the expression amounts were increased firstly and then decreased,but gradually disappeared in flowers.This study laid the foundation for further research on the function of Faapx-c gene.

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Last Update: 2016-07-14