|Table of Contents|

Effect of Light Quality and Light Intensity on the Chlorophyll Fluorescence Parameters of Red Raspberry Tissue Culture Plantlets(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年11
Page:
19-23
Research Field:
Publishing date:

Info

Title:
Effect of Light Quality and Light Intensity on the Chlorophyll Fluorescence Parameters of Red Raspberry Tissue Culture Plantlets
Author(s):
GUO Fang1; 2; WANG Chao3; LIU Haipeng1; 2; LI Baoguo1; 2; ZHANG Xuemei1; 2; QI Guohui1; 2
(1.College of Forestry，Agricultural University of Hebei，Baoding，Hebei 071000；2.Key Laboratory of Forest Resources and Forest Conservation in Hebei Province，Baoding，Hebei 071000；3.Beijing Double Large Agricultural Science and Technology Development Limited Company,Beijing 100091)
Keywords:
light quality; light intensity; red raspberry; chlorophyll fluorescence
PACS:
-
DOI:
10.11937/bfyy.201611005
Abstract:
In order to establish suitable light quality and light intensity for the growth of red raspberry tissue culture plantlets，using red raspberry ‘Heritage’ tissue culture plantlets as experimental material，red raspberry root chlorophyll content and chlorophyll fluorescence parameters of tissue culture plantlets of different light quality and light intensity were studied.The results showed that，chlorophyll a，chlorophyll b and the total chlorophyll content increased first and then decreased with increasing of R/B ratio except R/B treatment，chlorophyll a and the total chlorophyll content showed a maximum value in the treatment of 2R/B，respectively 1.344 mg?g-1，1.716 mg?g-1，chlorophyll b was the highest in R/2B，was 0.423 mg?g-1，chlorophyll a，chlorophyll b and the total chlorophyll content(Ct) were the lowest in 10R/B，respectively 0.130 mg?g-1，0.033 mg?g-1，0.163 mg?g-1，were significantly lower than other light；chlorophyll a/b were higher in 10R/B，2R/B and R/B，were 3.942，3.709，3.653，showing the characteristics of sun plants，and in 6R/B，W and 4R/B were lower，were 2.729，2.730，2.747,and showing the characteristics of shade plants；under R/2B，the chlorophyll fluorescence parameters of raspberry plantlets leaves showed the excellent value，the Fv/Fm value reached 0.881，however 10R/B，Fv/Fm decreased to 0.355，and significantly lower than the other light treatment.In light intensity treatment，chlorophyll a，chlorophyll b and the total chlorophyll content showed a decreasing trend with the increase of light intensity，respectively dropped to 0.907 mg?g-1，0.302 mg?g-1，1.209 mg?g-1 from the maximum value of 1.218 mg?g-1，0.503 mg?g-1，1.720 mg?g-1，the formation of significant differences，chlorophyll a/b was increased with the increase of light intensity，in the 2 000 lx reached the maximum value，was 3.042，and three kinds of light intensity were significantly different；the initial fluorescence Fo of leaves increased with the increase of light intensity，in the light intensity of 2 000 lx to a maximum value，was 180.000，maximum fluorescence values appeared in 1 500 lx，was 907.000，however,PSII of maximal chemical efficiency Fv/Fm，PSII potential activity of Fv/Fo，PSII actual photochemical efficiency of PSII，variable fluorescence decreased ratio Rfd were with the light intensity increasing，which presented a decreasing trend，in 1 000 lx were presented maximum，were 0.847，5.573，0.815，4.200,respectively.

References:

 

[1]尹相博,于咏梅,于立芝.红树莓组织培养研究进展[J].黑龙江农业科学,2013(2):140-143.

[2]刘卉,杨国伟,兰蓉,等.树莓叶中鞣花酸提取工艺的研究[J].食品研究与开发,2015,36(18):55-58.

[3]潘菊,杨双.双季树莓的组织培养及快速繁殖[J].北方园艺,2012(21):105-106.

[4]陈福明,陈顺伟.混合液法测定叶绿素含量的研究[J].林业科技通讯,1984(2):4-8.

[5]刘振威,贾文庆,刘会超,等.不同LED光源对银条组培苗不定芽生长的影响[J].北方园艺,2012(12):152-154.

[6]ANNA B，ALICJA K.Effect of light quality on somatic embryogenesis in Hyacinthus orientalis L.‘Delft’s blue’[J].Biological Bulletin of Poznan,2001,38(1):103-107.

[7]TANAKA M,TAKAMURA T,WATANABE H.In vitro growth of Cymbidium plantlets cultured under superbright red and blue light-emitting diodes(LEDs)[J].Journal of Horticultural Science and Biotechnology,1998,73(1):39-44.

[8]刘晓英,徐志刚,常涛涛,等.不同光质LED弱光对樱桃番茄植株形态和光合性能的影响[J].西北植物学报,2010,30(4):645-651.

[9]吴甘霖,段仁燕,王志高,等.干旱和复水对草莓叶片叶绿素荧光特性的影响[J].生态学报,2010,30(14):3941-3946.

[10]陈祥伟,刘世琦,王越,等.不同LED光源对乌塌菜生长、光合特性及营养品质的影响[J].应用生态学报,2014,25(7):1955-1962.

[11]黄俊,郭世荣,吴震,等.弱光对不结球白菜光合特性与叶绿体超微结构的影响[J].应用生态学报,2007(2):352-358.

[12]刘文海,高东升,束怀瑞.不同光强处理对设施桃树光合及荧光特性的影响[J].中国农业科学,2006(10):2069-2075.

[13]赵会杰,邹琦,于振文.叶绿素荧光分析技术及其在植物光合机理研究中的应用[J].河南农业大学学报,2000,34(3):248-251.

Memo

Memo:
-
Last Update: 2016-07-14