Effect of Different Concentration of Hormones on Dendrobium hercoglossum Rchb.f.Tissue Culture(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Effect of Different Concentration of Hormones on Dendrobium hercoglossum Rchb.f.Tissue Culture

Author(s):: WANG Yinuo; LI Linxuan; WEI Ying; LI Cui; XIAO Dong; WEI Kunhua; (Guangxi Botanical Garden of Medicinal Pant/Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement，Nanning，Guangxi 530023)

Keywords:: Dendrobium hercoglossum Rchb.f.; tissue culture; subculture; rooting culture

Abstract:: Dendrobium hercoglossum Rchb.f.spore was used as the explants and the MS was used as the basic culture medium，the effects of different hormone combinations on subculture，root culture were investigated.The results indicated that the best initial medium was MS+6-BA 1.5 mg?L-1+NAA 0.5 mg?L-1.The most effective medium for cluster inducing and subculture was MS+6-BA 2.0 mg?L-1+NAA 0.4 mg?L-1+KT 0.2 mg?L-1+AC 1.0 g?L-1.And the best rooting medium was MS+6-BA 0.5 mg?L-1+NAA 2.0 mg?L-1+AC 1.0 g?L-1.The average root length was 3.12 cm，and rooting ratio was above 90%.

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Last Update: 2016-07-12