Separation and Determination of Quinolizidine Aalkaloids in Callus of Sophora alopecuroides L.by HPLC and Its System Optimization(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Separation and Determination of Quinolizidine Aalkaloids in Callus of Sophora alopecuroides L.by HPLC and Its System Optimization

Author(s):: GAO Yuan1; LI Peng2; ZHANG Qingchen3; XU Quanzhi1; SUN Mudi1; GU Peiwen1; (1.Agricultural College，Ningxia University，Yinchuan，Ningxia 750021;2.Chemical Engineering College,Ningxia University,Yinchuan,Ningxia 750021;3.Pharmacy College,Shandong University,Jinan,Shandong 250100)

Keywords:: callus of Sophora alopecuroides L.; HPLC; oxysophocarpine; oxymatrine; sophoridine; sophocarpine; matrine

Abstract:: Taking the callus of Sophora alopecuroides L.as the experimental materials，using the method of HPLC for the separation and determination of oxysophocarpine，oxymatrine，sophoridine，sophocarpine and matrine in callus of Sophora alopecuroides L.,a chromatographic method for the separation and determination of five kinds of alkaloids from Sophora alopecuroides L.was established.The results showed that，chromatographic conditions,Ultimate  AQ-C18(4.6 μm×250 mm×5 mm)；mobile phase,0.01 mol?L-1 phosphate buffer(K2HPO4=5.59 g?L-1，KH2PO4=0.41 g?L-1，pH 7.5)-methanol(50∶50,V/V)；UV detection at 216 nm；column temperature,35 ℃;flow rate,1.0 mL?min-1.There were five kinds of alkaloids in the callus of Sophora alopecuroides L..The ingredients and contents of oxysophocarpine and oxymatrine were higher in subculture callus which were cultivated into stage 4.They were 1.205 6 mg?g-1 and 0.116 5 mg?g-1,sophoridine，sophocarpine and sophocarpidine were fluctuated slightly and downtrended more or less as the time went by.

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Last Update: 2016-07-11