|Table of Contents|

Molecular Determination of Seed Borne Viruses in Common Bean（Phaseolus vulgaris L.）(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年10
Page:
107-111
Research Field:
Publishing date:

Info

Title:
Molecular Determination of Seed Borne Viruses in Common Bean（Phaseolus vulgaris L.）
Author(s):
LI Chang1; FU Yali1; TAN Ying1; LU Hong1; FENG Guojun2; XU Qijiang1
（1.College of Life Sciences，Northeast Forestry University，Harbin，Heilongjiang 150040；2.Institute for Crop Research，Heilongjiang University，Harbin，Heilongjiang 150080）
Keywords:
common bean（Phaseolus vulgaris L.）; BCMV; BYMV; SBMV; PCR; RT-PCR
PACS:
-
DOI:
10.11937/bfyy.201610027
Abstract:
Bean common mosaic potyvirus (BCMV)，bean yellow mosaic virus (BYMV) and southern bean mosaic virus (SBMV) are the important seed borne pathogen of Heilongjiang common bean.For analyzing the status of common bean germplasm，taking the radicles，plumules，and cotyledons of six common bean seeds as materials，the genomic DNA and total RNA was extracted from different organs by CTAB method.Specific primers were designed according to published coat protein (CP) gene sequence of BCMV，BYMV and SBMV，respectively.180 germinated seeds of common bean were tested for the presence of the three viruses by polymerase chain reaction (PCR) and reverse transcription-polymerase chain reaction (RT-PCR).The results of RT-PCR revealed that the average infection frequencies of BCMV，BYMV，and SBMV in common bean samples were 12.78%，9.44% and 1.67%，respectively.Results of PCR revealed that the average infection frequencies of BCMV，BYMV and SBMV in common bean samples were 12.78%，8.33% and 1.67%，respectively.All six common bean varieties tested were positive one，two or three viruses.The causal viruses of common bean were BCMV and BYMV.

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Last Update: 2016-07-11