Using twenty kinds of different Agrocybe aegerita as the research objects which saved by Institute of Biology of Gansu Academy of Sciences and introduced from elsewhere.Different strains of Agrocybe were cultivated in the same planting material culture,in order to study the effect of the same material in different cultivated strains Agrocybe on mycelial growth,fruiting body biological traits,biological conversion of biology.The results showed that the biological character of AC9,AC22,AC24,AC26 had a better performance were the ideal cultivated strains.