Taking corn straw and soybean straw as the main test material，main index of Pleurotus citrinopileatus (mycelia growth rate,growth potential,contamination rate,commodity mushroom character,biological efficiency,input-output ratio) were measured,the effect of the two mixed straws on Pleurotus citrinopileatus mycelium and fruiting body growth were investigated，to evaluate the feasibility of the two kinds of straw mixed cultivation of Pleurotus citrinopileatus.The results showed that production indexes and economic benefit of Pleurotus citrinopileatus cultivated by corn straw and soybean straw were better,the two kinds of straw (corn straw 30％，soybean straw 69％,lime 1％) mixed cultivation Pleurotus citrinopileatus was feasible.